Original scientific paper

Purification and some Properties of Proteinases from Calf Thymus

T. Turnšek ; Department of Biochemistry, »J. Stefan« Institute, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
M. Kopitar ; Department of Biochemistry, »J. Stefan« Institute, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
I. Kregar ; Department of Biochemistry, »J. Stefan« Institute, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
D. Lebez ; Department of Biochemistry, »J. Stefan« Institute, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia

Abstract

Calf thymus was used for the investigation of intracellular
proteinases. The tissue was homogenized, centrifuged at a low
speed and applied on DEAE-cellulose. Active fractions were
collected and their proteolytic activity toward different protein
substrates was tested. It was found that several proteinases are
present in thymus; cathepsin D being the most abundant acid
proteinase. The activitiy at neutral pH was ascribed to fibrinogen
degrading proteinases.

Keywords

Hrčak ID:

197196

URI

https://hrcak.srce.hr/197196

Publication date:

20.3.1973.

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