Original scientific paper
https://doi.org/10.15255/CABEQ.2017.1181
Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis
B. Wang
; a) College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China; e) Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, China
Y. Pu
; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
H. G. Gerken
; Arizona Center for Algal Technology and Innovation, Arizona State University, Mesa, Arizona 85212, USA
Y. Xie
; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
L. Lin
; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China
H. Chen
; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Y. Lu
; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Abstract
D-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A
two-step culture strategy was employed to deal with the ambivalent culture conditions between the growth of Acetobacter tropicalis and the biosynthesis of D-GA. The first stage focuses on biomass accumulation with low initial glycerol concentration; the second
stage provides D-GA accumulation through whole-cell biocatalysis. Approximately 2-fold of D-GA yield and 4.5-fold of D-GA productivity were gained by the two-step culture strategy compared to the traditional fermentation with 150 g L–1 of glycerol. Our results also showed that A. tropicalis was able to catalyze the conversion of glycerol to D-GA in the presence of up to 10 % methanol. These findings have important implications to enhance D-GA yield by strategy optimization and reduce its cost.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
glyceric acid; Acetobacter; two-step culture; biocatalysis; fermentation; cost-effective production
Hrčak ID:
197253
URI
Publication date:
29.3.2018.
Visits: 1.196 *