Original scientific paper

https://doi.org/10.24099/vet.arhiv.170216

Determination of reference values for cytokines and matrix metalloproteinases in the synovial fluid of horses of different ages

Maria A. Chiappe Barbará ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Gustavo Perrone ; Equine Production Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Nicolás Caggiano ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Yael Lastra ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Florencia Rubatino ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Araceli Ferretto ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina
Emilio A. De Simone orcid.org/0000-0002-2481-0476 ; Animal Physiology Chair, School of Veterinary Sciences, Buenos Aires University, Buenos Aires, Argentina

Abstract

Osteoarthritis (OA) is the most common joint disease in sport horses. Inflammatory and anti-inflammatory
cytokines and metalloproteinases (MMPs) play a crucial role in the degradation of the cartilage extracellular
matrix. The study of inflammatory molecular biomarkers could be useful in the diagnosis and choice of
therapeutic strategies. However, little is known about the normal values of inflammatory biomarkers at different
ages. Moreover, early diagnosis of OA is crucial to ensure early treatment in order to prevent chronic damage.
The aim of this study was to establish a reference range for values of pro-inflammatory cytokines (IL-1β, IL-4,
IL-6 and TNF-α) and MMPs (MMP-2 and MMP-9) in the synovial fluid of horses of different ages. The old
horses group presented higher values of IL-4 and TNF-α than the rest of the groups studied (P<0.001 vs. all
other groups). IL-1β levels were higher in foals than in all the other groups (P<0.001). No statistically significant
differences were found between the groups as regards the mean IL-6 values. Synovial MMP-2 levels were
statistically lower in the old horses group (P<0.001) and higher in the foals group (P<0.05). Higher MMP-9
levels were found in the foals group (55.45 ± 58.1 percentage of proteolysis) (P<0.001 vs. all other groups).
This study demonstrates the existence of variations in the levels of pro-inflammatory markers in the synovial
fluid of horses at different ages

Keywords

matrix metalloproteinase; cytokines; osteoarthritis; synovial markers

Hrčak ID:

197838

URI

https://hrcak.srce.hr/197838

Publication date:

2.4.2018.

Article data in other languages: croatian

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