Original scientific paper

https://doi.org/10.20471/acc.2018.57.01.11

The Role of Pai-1 Gene 4g/5g Polymorphism and Diagnostic Value of Biomarkers in Allergic and Non-Allergic Asthma Phenotype

Marina Lampalo orcid.org/0000-0001-9264-6922 ; Jordanovac University Department for Lung Diseases, Zagreb University Hospital Centre, Zagreb, Croatia
Irena Jukić ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia; School of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Jasna Bingulac-Popović ; Department of Molecular Diagnosis, Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Hana Safić ; Department of Molecular Diagnosis, Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Nikola Ferara ; School of Medicine, University of Zagreb, Zagreb, Croatia
Sanja Popović-Grle ; Jordanovac University Department for Lung Diseases, Zagreb University Hospital Centre, Zagreb, Croatia

Abstract

Asthma is a chronic inflammatory disease that is characterized by reversible obstruction of airways, bronchial hyper-reactivity and airway remodeling. The etiology of asthma is multifactorial, with in-heritance playing an important role. The aim of our study was to investigate the importance of biomarkers of asthma and the role of plasminogen activator inhibitor-1 (PAI-1) gene as a genetic factor that could be involved in the pathogenesis of asthma. The research was conducted at Jordanovac University Department for Lung Diseases and Croatian Institute of Transfusion Medicine. The research included 149 patients with asthma and 89 healthy individuals. We collected demographic data of both study groups, determined asthma severity using GINA guidelines, and the values of biomarkers and PAI-1 by using laboratory techniques. Based on the results, we concluded that patients with allergic phenotype of asthma were younger, had better lung function and higher levels of IgE. By observing FeNO values, we were not able to distinguish asthmatic patients that had been diagnosed with obstruction of airways from asthmatic patients with normal lung function because FeNO indicates the inflammatory component of disease. The 4G/5G polymorphism of PAI-1 gene did not show any statistically significant difference in the distribution of 4G/4G, 4G/5G and 5G/5G between the group of asthmatic patients and control group.

Keywords

Asthma; Hypersensitivity; Biomarkers; Plasminogen activator inhibitor 1; Polymorphism, genetic

Hrčak ID:

201837

URI

https://hrcak.srce.hr/201837

Publication date:

1.3.2018.

Article data in other languages: croatian

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