Short communication, Note

https://doi.org/10.2478/acph-2018-0034

Pharmacological evaluation of phenylcarbamic acid derivatives on cardiovascular functions in rats

EVA KRALOVA ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Bratislava, Slovak Republic
EVA RACANSKA ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Bratislava, Slovak Republic
ANNA VICENOVA ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Bratislava, Slovak Republic
IVETA BOSELOVA ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Bratislava, Slovak Republic
IVAN MALIK ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Bratislava, Slovak Republic
TATIANA STANKOVICOVA ; Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Bratislava, Slovak Republic

Abstract

Four phenylcarbamic acid derivatives, 1 (1-(4-fluorophenyl)-4-[3-(4-methoxyphenylcarbamoyloxy)-2-hydroxypropyl]piperazinium chloride, 2 (1-(2-methylphenyl)-4-[3-(4-methoxyphenylcarbamoyloxy)-2-hydroxypropyl]piperazinium chloride), 3 (1-(2-methylphenyl)-4-[3-(4-ethoxyphenylcarbamoyloxy)-2-hydroxypropyl]piperazinium chloride) and 4 (1-(3-trifluoromethylphenyl)-4-[3-(4-methoxyphenylcarbamoyloxy)-2-hydroxypropyl]piperazinium chloride) were investigated for their ability to affect various cardiovascular functions and to establish their chemical structure-biological activity relationship. The compounds were evaluated for their antiarrhythmic efficacy using ouabain-induced rhythm disturbances and the ability to inhibit the positive chronotropic effect of isoproterenol in isolated atria of Wistar rats. Electrocardiogram (ECG) parameters in isolated hearts of spontaneously hypertensive rats (SHR) perfused according to the Langendorff method and ability to decrease phenylephrine-induced contraction of the aortic strips after repeated administration of the compounds were also analyzed. Only compound 3 delayed significantly the evaluated parameter of arrhythmogenicity and was able to antagonize the isoproterenol-induced positive chronotropic effect in normotensive rats’ atria. Similarly, in SHR rats, only compound 3 was able to decrease heart frequency significantly without influencing the duration of QT (time between the start of the Q wave and the end of the T wave) and QTc (frequency corrected QT) intervals. The evaluated endothelial function was improved after administration of compound 2. Fluorine-containing structures (1 and 4) were less effective compared to 2´-methylphenylpiperazine derivatives (2 and 3). The latter two compounds showed suitable efficacy, which supported their use for futher pharmacological research.

Keywords

phenylcarbamic acid derivatives; antiarrhythmic activity; isolated atria; electrical activity; vascular contractility; rat

Hrčak ID:

202265

URI

https://hrcak.srce.hr/202265

Publication date:

31.12.2018.

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