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Chemical and Biochemical Engineering Quarterly, Vol. 32 No. 2, 2018.

Original scientific paper

https://doi.org/10.15255/CABEQ.2016.978

Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate

H. Li ; a)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, China
L. Zhang ; a)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, China
R. Shen ; a)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, China
Y. Liu ; a)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, China

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Abstract

The synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB) was evaluated. The results showed excellent yield in the presence of Novozyme435 as catalyst, 10 % (w/w of l-ascorbic acid) of 4 Å molecular sieve and 10 % (w/w of l-ascorbic acid) of TBAB as additives in 1,4-dioxane. Furthermore, response surface methodology (RSM) and 5-level-4-factor central composite design (CCD) were employed to evaluate the effects of parameters on the yield of l-ascorbyl myristate. According to the
analysis result of ridge max, the optimal synthesis conditions were predicted, as follows: reaction time 27.1 h, temperature 54.1 °C, enzyme amount 25.9 % (% w/w of l-ascorbic acid), substrate molar ratio 5, and the optimal actual yield was 85.0 %.







This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

l-ascorbyl myristate; Novozyme435; TBAB; Response Surface Methodology

Hrčak ID:

203189

URI

https://hrcak.srce.hr/203189

Publication date:

5.7.2018.

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