Professional paper

https://doi.org/10.21860/medflum2018_203560

Genotyping of toxigenic strains of Clostridium difficile

Tanja Petrović ; Zavod za mikrobiologiju i molekularnu dijagnostiku, Sveučilišna klinička bolnica Mostar, Bosna i Hercegovina
Sanja Jakovac ; Zavod za mikrobiologiju i molekularnu dijagnostiku, Sveučilišna klinička bolnica Mostar, Bosna i Hercegovina

Abstract

Aim: The aim of this paper was the molecular analysis of toxigenic strains of C. difficile to determine the distribution of different genotypes.
Subjects and Methods: The study was performed on stools samples from patients hospitalized in the University Clinical Hospital Mostar. Production of toxins A and B was detected by ELFA technique in all clinical samples. Isolated C. difficile DNA was amplified by PCR reaction. Hybridization method was used for determinatin of: C. difficile specific gene (tpi), genes resposible for production of toxins A and B (tcdA, tcdB) and binary toxins (cdtA, cdtB), deletions in tcdC gene at positions 18 bp, 39 bp and 117 bp, and two different mutations in the gyrA gene.
Results: In all of the strains C. difficile, the toxin A and B genes (tcdA, tcdB) were present. Presence of the mutation in the gene for moxifloxacin (gyrA) resistance was demonstrated in 96.66% C. difficile strains. The same strains have also been shown to produce binary toxins, confirming that these strains belong to the hypervirulent ribotype 027.
Conclusions: This study has shown that in the University Clinical Hospital Mostar among toxigenic strains of C. difficile the predominant genotype was moxifloxacin-resistant, hypervirulent ribotype 027.

Keywords

Clostridium difficile; genotype; hypervirulent ribotype

Hrčak ID:

203560

URI

https://hrcak.srce.hr/203560

Publication date:

1.9.2018.

Article data in other languages: croatian

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