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Short communication, Note

A Quantitative Chromatographic Method for the Determination of Leucine Aminopeptidase Activity

D. Lebez ; Department of Radiobiology, Nuclear Institute »Joief Stefan«, Ljubljana, Slovenia, Yugoslavia
M. Funtek ; Department of Radiobiology, Nuclear Institute »Joief Stefan«, Ljubljana, Slovenia, Yugoslavia


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Abstract

A micro method for the determination of leucine aminopeptidase
activity was developed. Leucinamide was us ed as substrate. The
liberated leucin e was quantitative ly determined using paper chromatography
a nd reflectanc e-densitometrical scanning of the spots.
From the spot area of leucine the enzyme activity was evaluated.
This method is useful for the determination of leucine aminopeptidase
activity in supernatants obtained after centrifugation of
homogenized organs.

Keywords

Hrčak ID:

208077

URI

https://hrcak.srce.hr/208077

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