Original scientific paper

https://doi.org/10.2478/aiht-2018-69-3157

Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys

Dean Karaica ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Davorka Breljak ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Jovica Lončar ; Laboratory for Molecular Ecotoxicology, Ruđer Bošković Institute, Zagreb, Croatia
Mila Lovrić ; Clinical Institute of Laboratory Diagnosis, University Hospital Center, Zagreb, Croatia
Vedran Micek ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivana Vrhovac Madunić ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Hrvoje Brzica ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Carol M. Herak-Kramberger ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Jana Ivković Dupor ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marija Ljubojević ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Tvrtko Smital ; Laboratory for Molecular Ecotoxicology, Ruđer Bošković Institute, Zagreb, Croatia
Željka Vogrinc ; Clinical Institute of Laboratory Diagnosis, University Hospital Center, Zagreb, Croatia
Gerhard Burckhardt ; Institute for Systemic Physiology and Pathophysiology, University Medical Center Göttingen, Göttingen, Germany
Birgitta C. Burckhardt ; Institute for Systemic Physiology and Pathophysiology, University Medical Center Göttingen, Göttingen, Germany
Ivan Sabolić orcid.org/0000-0002-2587-9109 ; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia

Abstract

Chloride/formate exchanger (CFEX; SLC26A6) mediates oxalate transport in various mammalian organs. Studies in Cfex knockout mice indicated its possible role in development of male-dominant hyperoxaluria and oxalate urolithiasis. Rats provide an important model for studying this pathophysiological condition, but data on Cfex (rCfex) localisation and regulation in their organs are limited. Here we applied the RT-PCR and immunochemical methods to investigate rCfex mRNA and protein expression and regulation by sex hormones in the pancreas, small intestine, liver, and kidneys from intact prepubertal and adult as well as gonadectomised adult rats treated with sex hormones. rCfex cDNA-transfected HEK293 cells were used to confirm the specificity of the commercial anti-CFEX antibody. Various biochemical parameters were measured in 24-h urine collected in metabolic cages. rCfex mRNA and related protein expression varied in all tested organs. Sex-independent expression of the rCfex protein was detected in pancreatic intercalated ducts (apical domain), small intestinal enterocytes (brush-border membrane; duodenum > jejunum > ileum), and hepatocytes (canalicular membrane). In kidneys, the rCfex protein was immunolocalised to the proximal tubule brush-border with segment-specific pattern (S1=S2

Keywords

anion exchanger; immunolocalisation; nephrolithiasis; oxaluria; RT-PCR; sex differences; urolithiasis

Hrčak ID:

213621

URI

https://hrcak.srce.hr/213621

Publication date:

20.12.2018.

Article data in other languages: croatian

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