Original scientific paper

https://doi.org/10.24099/vet.arhiv.0229

In vitro and in vivo assessment of orf virus (ORFV) by electron microscopy

Nawab Nashiruddullah orcid.org/0000-0003-4902-994X ; Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
Debesh C. Pathak ; Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
Nagendra N. Barman ; Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
Jafrin A. Ahmed orcid.org/0000-0002-3702-4255 ; Division of Veterinary Physiology and Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, RS Pura, Jammu and Kashmir, India
Probodh Borah ; State Biotechnology Hub, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
Safeeda S. Begum ; Department of Veterinary Medicine, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
Saidul Islam ; Department of Veterinary Parasitology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India

Abstract

Transmission electron microscopy (TEM) was employed for describing skin and scab lesions in goats affected by orf virus and to demonstrate the parapoxvirus from clinical suspensions by negative staining and ORFV confirmation by immunogold electron microscopy. All samples were confirmed as parapoxvirus by semi-nested PCR amplification of partial gene encoding for the B2L envelope protein. Skin lesions were characterized by ballooning degeneration and loss of desmosomes of the spinosum cells, cytolysis and vesicle formation. Nuclear changes included chromatin margination and increase in electron density. Cytoplasmic changes were typical of cell swelling, vacuolation and the presence of uniform, moderately electron dense viroplasm, situated in the perinuclear region. Various intracellular forms including immature virions (IV), mature virions (IMV) and wrapped virions (WV) were observed in the cytoplasm. All these forms of ORFV were observed morphologically akin to vaccinia virus (VACV). Negative staining of clinical samples and viral suspensions showed typical parapoxvirus morphology with a characteristic criss-cross tubular surface pattern. Both ‘capsule’ form (‘C’ form) and ‘mulberry’ form (‘M’ form) of the virus were demonstrated. ORFV speciation was confirmed by immunogold tagging using a monoclonal antibody in ultra-thin skin section.

Keywords

goat; gold labelling; orf virus; electron microscopy; parapoxvirus

Hrčak ID:

214769

URI

https://hrcak.srce.hr/214769

Publication date:

15.12.2018.

Article data in other languages: croatian

Visits: 1.549 *