Original scientific paper

Risk factors and molecular predispositions for cervical dysplasia among women from east Croatia

Magdalena Perić orcid.org/0000-0002-5237-9329 ; Microbiology Department, Institute of Public Health Osijek-Baranja County, Croatia
Zinka Bošnjak ; Department of Transfusion Medicine, University Josip Juraj Strossmayer, University Hospital Centre Osijek, Croatia
Snježana Džijan ; Department of Transfusion Medicine, University Josip Juraj Strossmayer, University Hospital Centre Osijek, Croatia
Dinko Paulić orcid.org/0000-0001-9890-2755 ; Microbiology Department, Institute of Public Health Osijek-Baranja County, Croatia
Ivana Roksandić-Križan orcid.org/0000-0002-3841-2751 ; Microbiology Department, Institute of Public Health Osijek-Baranja County, Croatia
Ivona Djurkin-Kušec orcid.org/0000-0002-1363-8447 ; Faculty of Agriculture in Osijek, University Josip Juraj Strossmayer, Osijek, Croatia
Maja Bogdan ; Microbiology Department, Institute of Public Health Osijek-Baranja County, Croatia, Faculty of Medicine in Osijek, University Josip Juraj Strossmayer, Osijek, Croatia
Dubravka Vuković ; Microbiology Department, Institute of Public Health Osijek-Baranja County, Croatia
Stjepan Rudan orcid.org/0000-0001-7865-0415 ; Faculty of Dental Medicine and Health, University Josip Juraj Strossmayer, Osijek, Croatia

Abstract

Purpose: The aim of this study was to investigate possible association between high-risk Human papillomavirus (HR HPV) – induced cervical infection, HR HPV-related cervical dysplasia, HR HPV genotypes with two Toll-like receptor (TLR) 9 gene polymorphisms and other risk factors.
Methods: During a three-year period, 100 women positive for cervical HR HPV infection (97 with cervical dysplasia and 3 positive women without dysplasia) were genotyped using the Linear Array HPV Genotyping assay (Roche Diagnostics). Furthermore, two polymorphisms of TLR9 (-1486T/C, rs187084 and 2848C/T rs352140) were determined using real-time Polymerase Chain Reaction; 50 HR HPV negative women of similar ethnicity were included as controls.
Results: This study showed that infections with HPV 16 in women with cervical dysplasia were more frequently found compared with HPV 18 infections
(p=0.0539). Comparison between HR HPV positive and negative women showed significant association between age >35 years (p=0.0058), being unmarried
women (p=0.0001), nocondom usage (p=0.0304) and active tobacco smoking (p=0.0376) with HR HPV cervical infection. No significant associations between two TLR9 gene polymorphisms, HR HPV infection and cervical dysplasia were found.
Conclusion: Our results indicated that: i) women with cervical dysplasia showed significant higher rate of HR HPV 16 infection compared to HR HPV 18, ii) HR HPV – infection was strongly correlated with social risk factors and iii) TLR9 gene polymorphisms (rs187084; rs352140) did not correlate with HR HPV infection and cervical dysplasia. Further genome-wide association studies could open new frontier in understanding the relationship between polymorphisms at TLR9 and immunological mechanisms in HPV-induced carcinogenesis.

Keywords

Carcinogenesis; Human papillomavirus 16; Human papilloma virus 18; Toll-Like Receptor 9; Uterine Cervical Dysplasia

Hrčak ID:

220028

URI

https://hrcak.srce.hr/220028

Publication date:

13.5.2019.

Article data in other languages: croatian

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