Professional paper
Modifying ability for biofilm formation by clinical isolates of gram-negative microorganisms under negative pressure conditions in vitro
Valentine Kovalchuk
orcid.org/0000-0002-3351-2390
; Department of Microbiology, Vinnytsia National Medical University, Vinnytsia, Ukraine
Viacheslav Kondratiuk
orcid.org/0000-0002-2316-7941
; Department of Microbiology, Vinnytsia National Medical University, Vinnytsia, Ukraine
Olena Kondratiuk
orcid.org/0000-0002-3351-2390
; Military Medical Clinical Center of Central Region, Vinnytsia, Ukraine
Abstract
Vacuum-assisted closure therapy has been utilized as a ubiquitous wound management resource. Current studies of the subject focus on the influence of negative pressure on the bacterial load of wound bed and the possibility to remove matured biofilm, however, its impact on the ability of microorganisms to activate or reduce the biofilm producing modality remains unexplored. The goal of the present study was to evaluate the potential effect of negative pressure on biofilm producing modality of gram-negative rods with regard of initial biofilm positive or biofilm negative phenotype. Biofilm formation was evaluated for the strains of A. baumannii, P. aeruginosa, E. cloace, K. pneumonia and P. mirabilis isolated from infected war wounds of the extremities. The changes of optical density of the biofilm produced by the same strain under normal and negative pressure conditions, as well as the number of strains, which modified their ability to biofilm production, were measured.
The strains with initial biofilm negative phenotype under negative pressure switched to biofilm producing more vigorously than biofilm positive ones regardless of their taxonomical belonging. Pseudomonas strains demonstrate the highest rate of induction to biofilm producing under negative pressure conditions among all studied microorganisms.
Thus, the activation of biofilm producing mechanisms under negative pressure could be considered as a protective strategy, which helps ensure persistence of microorganisms in the wound even in case the vacuum-assisted closure therapy is applied.
Keywords
biofilm formation; vacuum-assisted closure
Hrčak ID:
220029
URI
Publication date:
13.5.2019.
Visits: 1.381 *