Short communication, Note

https://doi.org/10.2478/acph-2020-0013

Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed

BARBARA KRÓL-KOGUS ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, Poland
DANIEL GŁÓD ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, Poland
MIROSŁAWA KRAUZE-BARANOWSKA ; Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, Poland

Abstract

Fenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplements ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSD-ESI-MS method. Two C-18 columns connected in a series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminary validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %.

Keywords

fenugreek seeds; saponins; HPLC-ELSD-ESI-MS; qualitative and quantitative analysis

Hrčak ID:

220883

URI

https://hrcak.srce.hr/220883

Publication date:

31.3.2020.

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