Acta Pharmaceutica, Vol. 70 No. 1, 2020.
Short communication, Note
https://doi.org/10.2478/acph-2020-0016
Sildenafil alters biogenic amines and increases oxidative damage in brain regions of insulin-hypoglycemic rats
DAVID CALDERÓN GUZMÁN
; Laboratory of Neurosciences, Instituto Nacional de Pediatría (INP), Mexico
NORMA OSNAYA BRIZUELA
; Laboratory of Neurosciences, Instituto Nacional de Pediatría (INP), Mexico
MARIBEL ORTÍZ HERRERA
; Laboratory of Experimental Bacteriology, INP, Mexico
ARMANDO VALENZUELA PERAZA
; Laboratory of Neurosciences, Instituto Nacional de Pediatría (INP), Mexico
GERARDO BARRAGÁN MEJÍA
; Laboratory of Neurosciences, Instituto Nacional de Pediatría (INP), Mexico
HUGO JUÁREZ OLGUÍN
; Laboratory of Pharmacology, INP, Mexico; Department of Pharmacology, Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico
FRANCISCA TRUJILLO JIMÉNEZ
; Laboratory of Pharmacology, INP, Mexico
Abstract
The aim of the present study was to determine the effect of sildenafil on dopamine, 5-hydroxyindol acetic acid (5-HIAA) and selected biomarkers of oxidative stress in the brain of hypoglycemic rats. The animals were treated intraperitoneally as follows: group 1 (control), saline solution; group 2, insulin (10 U per rat or 50 U kg–1); group 3, insulin + single dose of sildenafil (50 U kg–1 + 50 mg kg–1); group 4, insulin + three doses of sildenafil every 24 hours (50 U kg–1 + 50 mg kg–1). In groups 2, 3 and 4, insulin was administered every 24 hours for 10 days. Blood glucose was measured after the last treatment. On the last day of the treatment, the animals´ brains were extracted to measure the levels of oxidative stress markers [H2O2, Ca2+,Mg2+-ATPase, glutathione and lipid peroxidation (TBARS)], dopamine and 5-HIAA in the cortex, striatum and cerebellum/medulla oblongata by validated methods. The results suggest that administration of insulin in combination with sildenafil induces hypoglycemia and hypotension, enhances oxidative damage and provokes changes in the brain metabolism of biogenic amines. Administration of insulin and sildenafil promotes biometabolic responses in glucose control, namely, it induces hypoglycemia and hypotension. It also enhances oxidative damage and provokes changes in the brain metabolism of biogenic amines.
Keywords
sildenafil; rat; hypoglycemia; biogenic amines; glutathione; H2O2; lipid peroxidation
Hrčak ID:
220886
URI
Publication date:
31.3.2020.
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