Original scientific paper

https://doi.org/10.11613/BM.2019.020708

Effect of temperature and time delay in centrifugation on stability of select biomarkers of nutrition and non-communicable diseases in blood samples

Ransi Ann Abraham ; Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Delhi, India
Praween K. Agrawal ; UNICEF, Delhi, India
Rajib Acharya ; Population Council, Delhi, India
Avina Sarna ; Population Council, Delhi, India
Sowmya Ramesh ; Population Council, Delhi, India
Robert Johnston ; UNICEF, Delhi, India
Arjan de Wagt ; UNICEF, Delhi, India
Nizamuddin Khan ; Population Council, Delhi, India
Akash Banerjee Kurundkar4, Arvind Pandey5, Raghu Pullakhandam6, Krishnapillai M Porwal ; Population Council, Delhi, India
Sucheta Banerjee Kurundkar ; Clinical Development Services Agency, Translational Health Science & Technology Institute, Faridabad, Haryana
Arvind Pandey ; Ex Director, National Institute of Medical Statistics, Indian Council of Medical Research, Delhi, India
Raghu Pullakhandam ; National Institute of Nutrition, Hyderabad, Telangana, India
Krishnapillai Madhavan Nair ; National Institute of Nutrition, Hyderabad, Telangana, India
Geeta Trilok Kumar ; Institute of Home economics, Delhi, India
H P S Sachdev ; Senior Consultant, Paediatrics and Clinical Epidemiology, B-16 Qutab Institutional Area, New Delhi, India
Umesh Kapil ; Department of Human Nutrition, All India Institute of Medical Sciences, Delhi, India
Renu Saxena ; Department of Human Nutrition, All India Institute of Medical Sciences, Delhi, India
Sila Deb ; Department of Hematology, All India Institute of Medical Sciences, Delhi, India
Ajay Khera ; Ministry of Health and Family Welfare, Delhi, India
Lakshmy Ramakrishnan ; Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Delhi, India

Abstract

Introduction: Preanalytical conditions are critical for blood sample integrity and poses challenge in surveys involving biochemical measurements.
A cross sectional study was conducted to assess the stability of select biomarkers at conditions that mimic field situations in surveys.
Material and methods: Blood from 420 volunteers was exposed to 2 – 8 °C, room temperature (RT), 22 – 30 °C and > 30 °C for 30 min, 6 hours, 12
hours and 24 hours prior to centrifugation. After different exposures, whole blood (N = 35) was used to assess stability of haemoglobin, HbA1c and
erythrocyte folate; serum (N = 35) for assessing stability of ferritin, C-reactive protein (CRP), vitamins B12, A and D, zinc, soluble transferrin receptor
(sTfR), total cholesterol, high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL), tryglicerides, albumin, total protein
and creatinine; and plasma (N = 35) was used for glucose. The mean % deviation of the analytes was compared with the total change limit (TCL),
computed from analytical and intra-individual imprecision. Values that were within the TCL were deemed to be stable.
Result: Creatinine (mean % deviation 14.6, TCL 5.9), haemoglobin (16.4%, TCL 4.4) and folate (33.6%, TCL 22.6) were unstable after 12 hours at 22-
30°C, a temperature at which other analytes were stable. Creatinine was unstable even at RT for 12 hours (mean % deviation: 10.4). Albumin, CRP,
glucose, cholesterol, LDL, triglycerides, vitamins B12 and A, sTfR and HbA1c were stable at all studied conditions.
Conclusion: All analytes other than creatinine, folate and haemoglobin can be reliably estimated in blood samples exposed to 22-30°C for 12 hours
in community-based studies.

Keywords

survey; preanalytical conditions; temperature; time delay; centrifugation

Hrčak ID:

221080

URI

https://hrcak.srce.hr/221080

Publication date:

15.6.2019.

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