Original scientific paper
https://doi.org/10.5513/JCEA01/20.2.2136
Aloenzimna genetichna harakteristika na pchelni semeystva Apis mellifera (Hymenoptera: Apidae) ot Bulgaria s razlichno higienno povedenie
Svilen Lazarov
; Trakia University, Faculty of Agriculture, Department of Animal Science-Nonruminants and other Animals, Section of Apiculture and Sericulture
Ivan Stoyanov
; Plovdiv University “Paisii Hilendarski”, Biological Faculty, Department of Developmental Biology, Section of Genetics
Vida Georgieva
; Plovdiv University “Paisii Hilendarski”, Biological Faculty, Department of Developmental Biology, Section of Genetics
Ivanka Zhelyazkova
; Trakia University, Faculty of Agriculture, Department of Animal Science-Nonruminants and other Animals, Section of Apiculture and Sericulture
Evgeniya Ivanova
; Plovdiv University “Paisii Hilendarski”, Biological Faculty, Department of Developmental Biology, Section of Genetics
Abstract
Obekt na nastoyashtoto izsledvane e genetichnata izmenchivost, ustanovena pri rabota s 25 pchelni semeystva ot razlichni rayoni na Bulgaria s razlichno higienno povedenie (Visoko higienichni, higienichni i ne-higiennichni). Izyaveniyat polimorfizam e harakteriziran na bazata na aloenzimen analiz po dve sistemi (MDH-1 i Est-3), saotvetstvashti na dva polimorfni lokusa. Obshto 1150 pcheli rabotnichki sa vklyucheni v izsledvaneto. Ustanoveno e, che MDH-1 lokusat e polimorfen vav vsichki izsledvani pchelni semeystva i e predstaven ot dva alela - MDH-165 i MDH-1100. Est-3 lokusat e fiksiran v deset ot izsledvanite kolonii. Po tozi lokus e konstatiran polimorfizam s prisastvie obshto na chetiri alelni varianti (Est-380, Est-388, Est-3100 i Est-3118) za genofonda na ostanalite pchelni semeystva. Izchisleniyat polimorfizam e mezhdu 50% i 100%. Ustanovenata i ochakvanata heterozigotnost (Ho i He) varira saotvetno ot 0.296 do 0.354 i 0.28 ot 0.332 pri ne-higienichnite i visoko higienichnite grupi. Izchislenite sredna nablyudavana i ochakvana heterozigotnosti sa saotvetno 0.32 i 0.307. Izchislenite niva na Fst i Nm pokazvat po-niska diferentsiatsia mezhdu visoko higienichnite i higienichnite semeystva i visoka diferentsiatsia mezhdu visoko higienichnite i ne-higienichnite semeystva. Konstatirani i obsadeni sa razlichia mezhdu nivata na polimorfizam, heterozigotnost, Fst, Nm i alelnite chestoti v izsledvanite grupi pchelni semeystva s razlichno higienno povedenie. Rezultatite ot nastoyashtoto prouchvane davat nova informatsia otnosno zavisimostta mezhdu nivata na higienno povedenie i aloenzimnite harakteristiki, koito biha mogli da se izpolzvat v badeshti deynosti po selektsia na medonosnite pcheli v Bulgaria.
Keywords
aloenzimi; medonosna pchela; genetichen polimorfizam; higienno povedenie
Hrčak ID:
221564
URI
Publication date:
28.6.2019.
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