Meeting abstract
Pentadecapeptide BPC 157 therapy in rats with cysteamine induced-terminal ileitis
Mariam Samara
; Department of Pharmacology, School of medicine University of Zagreb
Ivan Krezić
; Department of Pharmacology, School of medicine University of Zagreb
Marko Antunović
; Department of Pharmacology, School of medicine University of Zagreb
Marko Belamarić
; Department of Pharmacology, School of medicine University of Zagreb
Will Migo
; Department of Pharmacology, School of medicine University of Zagreb
Slaven Gojković
; Department of Pharmacology, School of medicine University of Zagreb
Vilim Dretar
; Department of Pharmacology, School of medicine University of Zagreb
Abstract
We introduce pentadecapeptide BPC 157 therapy in rats with cysteamine induced-terminal ileitis 1h/1month/2months. We counteracted gross hyperemia, edema, erosion, bleeding, microscopically significant loss of villous architecture, loss and shortening of villae and severe lymphocytic infiltrate. Pentadecapeptide counteracts various lesions in the whole GI-tract and free radical formation, and tested in ulcerative colitis trials and now in multiple sclerosis. Cysteamine was known to induce gastric-acid hypersecretion as a prototype of duodenal lesion. Cysteamine induced duodenal lesions after gastrectomy, and applied as an enema, ulcerative colitis in rats Cysteamine was applied in female Albino Wistar rats into the terminal ileum, 5 cm segment up to ileocecal valve, which was kept gently compressed for 1 min, and then released. Medication(BPC, or saline (controls)) was applied as an abdominal bath immediately after the end of the cysteamine application procedure, and then if rats were not sacrificed at 1 h, continuously, perorally in drinking water till the end of 1 or 2 months The hyperemia, edema, erosion and bleeding scores were summarized. Microscopically, cysteamine induced terminal ileitis presents with: submucosal congestion, significant loss of villous architecture, loss and shortening of villae and lamina propria infiltrated with mild to severe lymphocytic infiltrate, much like intraepithelial lymphocyte infiltration and some epithelial elevation from lamina propria. Better preservation of mucosal architecture appears in pentadecapeptide treated rats. There is only mild villous edema with capillary congestion and mild lymphocytic infiltrate. No epithelial elevation from lamina propria For further therapy, beneficial effect of the BPC counteracts cysteamine- terminal ileitis.
Keywords
cysteamine; terminal ileitis; BPC 157
Hrčak ID:
222569
URI
Publication date:
9.4.2019.
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