Original scientific paper

A preliminary study of Chlamydophila felis prevalence among domestic cats in the City of Zagreb and Zagreb County in Croatia

Gordana Gregurić Gračner ; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Vlahović Ksenija ; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Alenka Dovč ; Faculty of Veterinary Medicine, University of Ljubljana, Slovenia
Brigita Slavec ; Faculty of Veterinary Medicine, University of Ljubljana, Slovenia
Ljiljana Bedrica ; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Slavko Žužul ; Faculty of Veterinary Medicine, University of Zagreb, Croatia
Damjan Gračner ; Faculty of Veterinary Medicine, University of Zagreb, Croatia

Abstract

This research was preliminary study of the presence and prevalence of Chlamydophila felis (Cp. felis) among domestic cats in the City of Zagreb and Zagreb County in Croatia. 48 pure and mixed breed cats were examined. 192 conjunctival and nasal swab samples were examined using a rapid immunoenzyme assay (EIA). 30 conjunctival scraping samples of 15 cats that were EIA positive were examined using DIF. Four of them were positive, while the results of two samples were inconclusive. These six samples (three nostril samples and three eye samples) were examined using a conventional polymerase chain reaction (PCR), and only two samples from the same cat were found to be positive for Cp. felis. The sequencing of the PCR product confirmed that the causative agent of the cat’s conjunctivitis was Cp. felis. By using direct immunofluorescence as well as a polymerase chain reaction procedure and sequencing, the results of the research established the existence of the bacteria Cp. felis in one out of 48 domestic cats.

Keywords

feline chlamydiosis; cat; Chlamydophila felis; immunofluorescence; PCR; Zagreb; Zagreb County

Hrčak ID:

222772

URI

https://hrcak.srce.hr/222772

Publication date:

11.2.2018.

Article data in other languages: croatian

Visits: 1.532 *