Original scientific paper
https://doi.org/10.5513/JCEA01/20.3.2175
Evaluation of genetic stability of sugar beet (Beta vulgaris L.) plants obtained from unfertilized ovules using RAPD markers
Magdalena Tomaszewska-Sowa
; University of Technology and Life Sciences in Bydgoszcz, Department of Agricultural Biotechnology, Bernardyńska 6, 85-029 Bydgoszcz Poland
Dorota Olszewska
; University of Technology and Life Sciences in Bydgoszcz, Department of Agricultural Biotechnology, Prof. S. Kaliskiego 7, 85-789 Bydgoszcz, Poland
Abstract
In order to improve methods of embryo regeneration applied so far in in vitro cultures, a two-phased, innovative method involving liquid and solid media was used. Regeneration of ovules with the application of the gynogenesis technique takes place mainly through indirect organogenesis, which involves the formation of callus and the possibility of somaclonal variation. In the research, the DNA was isolated from the leaves of 10 regenerants, previously rooted and planted into the field. 5 of them were characterized by a haploid number of chromosomes whereas the others were of diploid nature. As a control, DNA originating from the leaves of a haploid plant growing in in vitro cultures on growth regulator-free MS medium was used. Plants regenerated on media of various quantitative and qualitative composition in terms of phytohormonal characteristic. The results of the experiment showed that 9 out of 19 tested primers generate polymorphic bands while the remaining 10 amplify the same DNA fragments in all tested samples. The polymorphism effect was revealed in 4 plants with diploid genome and in 1 haploid plant. In total, 13 polymorphic bands were identified and 11 of them were detected in diploid DNA.
Keywords
gynogenesis; PCR; RAPD; somaclonal variation; sugar beet
Hrčak ID:
225155
URI
Publication date:
10.9.2019.
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