Acta Pharmaceutica, Vol. 69 No. 4, 2019.
Original scientific paper
https://doi.org/10.2478/acph-2019-0049
Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres
MARIETA DUVNJAK ROMIĆ
; R&D, PLIVA Croatia Ltd, TEVA Group Member, Zagreb, Croatia
ANJA SUŠAC
; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia
JASMINA LOVRIĆ
; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia
BISERKA CETINA-ČIŽMEK
; R&D, PLIVA Croatia Ltd, TEVA Group Member, Zagreb, Croatia
JELENA FILIPOVIĆ-GRČIĆ
; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia
ANITA HAFNER
; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia
Abstract
The aim of this study was to evaluate long-term stability and assess the wound healing potential of the innovative melatonin-loaded lipid-enriched hybrid system compared to conventional melatonin-loaded chitosan microspheres. The hybrid system contained nanostructured lipid carrier incorporated in the chitosan matrix, in order to modify melatonin release and alter physicochemical characteristics of the delivery system. Stability testing was performed during a six-month period under two conditions - refrigerated (5±3 °C) and at room temperature (25 ± 2 °C/60 ± 5 % RH). Samples stored at both conditions were analyzed in terms of particle size, zeta potential, moisture content and thermal properties. At the end of testing, drug content was determined in all samples. Dressings wound healing potential was assessed by in vitro scratch test using human skin fibroblast cell line. Although both systems showed good stability characteristics, the addition of lipids in the system has improved its wound healing potential.
Keywords
melatonin; nanoparticles; microspheres; chitosan; stability; wound healing
Hrčak ID:
225427
URI
Publication date:
31.12.2019.
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