Original scientific paper
https://doi.org/10.2478/cjf-2019-0012
DIETARY ADMINISTRATION OF ETHANOL AND METHANOL EXTRACTS OF Withania somnifera ROOT STIMULATES INNATE IMMUNITY, PHYSIOLOGICAL PARAMETERS AND GROWTH IN NILE TILAPIA Oreochromis niloticus
Debosree Mukherjee
; Department of Zoology, University of Calcutta, Kolkata, India Fish Endocrinology Research Unit, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata – 700019, West Bengal, India
Indranath Ghosal
Mahammed Moniruzzaman
Manojit De
Suman Bhusan Chakraborty
Abstract
Efficacy of ethanol and methanol extracts of medicinal herb Withania somnifera roots was evaluated in Nile tilapia Oreochromis niloticus on immunostimulation and growth. Withania roots were extracted with water, ethanol, methanol, methylene chloride, hexane, successive methanol. Phenol, flavonoid content and antiradical activity of all the extracts weremeasured. Ethanol extract showed the highest phenol, flavonoid content and antiradical activity followed by methanol extract. Subsequently, Nile tilapia (n = 126) were fed diets containing different concentrations (0.0, 0.3, 0.5 and 0.7 g kg-1 feed) of ethanol and methanol extracts of W. somnifera roots for 30 days (3 replicates). Fish fed plant extract fortified diets showed significantly better (P<0.05) immunological, haematological, biochemical and growth parameters compared to the fish fed control diet. Fish fed dietcontaining ethanol extract at the concentration of 0.7 g kg-1 feed showed the highest immunological (phagocytotic activity, respiratory burst activity, serum lysozyme, total protein, total immunoglobulin), haematological (total red blood cells, haemoglobin, hematocrit, total white blood cells, lymphocyte), biochemical (reduced glutathione, glutathione reductase activity) and growth (final weight, weight gain, daily weight gain, specific growth rate) parameters. The plant extract might act as potent free radical scavenger in fish tissues and have tissue protecting ability, thus increasingfish health.
Keywords
ethanol extract; methanol extract; immunostimulation; growth; antioxidant
Hrčak ID:
225885
URI
Publication date:
30.9.2019.
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