Original scientific paper

https://doi.org/10.11613/BM.2019.030702

Determining biological variation of serum parathyroid hormone in healthy adults

Müjgan Ercan ; Faculty of Medicine, Department of Biochemistry, Harran University, Şanlıurfa, Turkey
Emiş Deniz Akbulut ; Biochemistry Laboratory, University of Health Sciences, Ankara Child Health and Diseases Hematology Oncology Training and Research Hospital, Ankara, Turkey
Esin Avcı ; Faculty of Medicine, Department of Biochemistry, Pamukkale University, Denizli, Turkey
Çiğdem Yücel ; Biochemistry Laboratory, Ankara Numune Training and Research Hospital, Ankara, Turkey
Esra Fırat Oğuz ; Biochemistry Laboratory, Ankara Numune Training and Research Hospital, Ankara, Turkey
Turan Turhan ; Biochemistry Laboratory, Ankara Numune Training and Research Hospital, Ankara, Turkey
Muhittin Serdar ; Faculty of Medicine, Department of Biochemistry, Acıbadem University, İstanbul, Turkey

Abstract

Introduction: Measurement of parathyroid hormone (PTH) is essential in the investigation and management of calcium metabolism disorders. To
assess the significance of any assay result when clinical decision making biological variation (BV) of the measurand must be taken into consideration. The aim of the present study is determining the BV parameters for serum PTH.
Materials and methods: Blood samples were taken at weekly intervals from 20 healthy subjects for ten weeks in this prospective BV study. Serum “intact PTH” concentrations were measured with electrochemiluminescence method. Biological variation parameters were estimated using the approach proposed by Fraser.
Results: The values of within-subject biological variation (CVI), between-subject biological variation (CVG), analytical variation (CVA), reference change value (RCV) and individuality index (II) for serum PTH were 21.1%, 24.9%, 3.8%, 59.4% and 0.8%, respectively. Within-subject biological variation and CVG were also determined according to gender separately; 18.5% and 24.0%; 26.2% and 18.6% for male and female, respectively. Calculated desirable precision and bias goals were < 10.6% and < 6.3%, respectively.
Conclusion: This study may contribute to BV data on serum PTH as it includes a sufficient number of volunteers from both genders over an acceptable period of time. We do not recommend the usage of population-based reference intervals for serum PTH concentrations. Reference change value may be helpful for the evaluation of serial serum PTH results. Nonetheless, evaluation of data according to gender is necessary when setting analytical performance specifications.

Keywords

biological variation; individuality; reference change value; PTH; quality specification

Hrčak ID:

226445

URI

https://hrcak.srce.hr/226445

Publication date:

15.10.2019.

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