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Acta Pharmaceutica, Vol. 70 No. 3, 2020.

Original scientific paper

https://doi.org/10.2478/acph-2020-0028

Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS

RABAB M. SOLIMAN ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, El Arish, North Sinai, Egypt
RANDA A. ABDEL SALAM ; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
BASMA G. EID ; Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
AHDAB KHAYYAT ; Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
THIKRYAT NEAMATALLAH ; Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
MOSTAFA K. MESBAH ; Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
GHADA M. HADAD ; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

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Abstract

The aim of this study was to investigate the stability of three major antioxidants of Nigella sativa: thymoquinone (TQ), carvacrol (CR) and thymol (THY), under different stress conditions using HPLC and LC-MS/MS. Forced degradation for each compound was performed under different conditions, including oxidation, hydrolysis, photolysis and thermal decomposition. The results showed that both CR and THY were stable under the studied conditions, whereas TQ was not affected by acidic, basic and oxidative forced conditions but the effect of light and heat was significant. The degradation products of TQ were further investigated and characterized by LC-MS/MS. HPLC-UV method has been fully validated in terms of linearity and range, the limit of detection and quantitation, precision, selectivity, accuracy and robustness. The method was successfully applied to quantitative analysis of the principal antioxidants of Nigella sativa TQ, CR and THY in different phytopharmaceuticals.

Keywords

thymoquinone; carvacrol; thymol; HPLC-UV; LC-MS/MS; stability

Hrčak ID:

226642

URI

https://hrcak.srce.hr/226642

Publication date:

30.9.2020.

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