Short communication, Note

https://doi.org/10.2478/v10007-008-0003-6

Studying the formation of aggregates in recombinant human granulocyte-colony stimulating factor (rHuG-CSF), lenograstim, using size-exclusion chromatography and SDS-PAGE

JASMINA TONIC RIBARSKA orcid.org/0000-0002-0026-3252 ; Faculty of Pharmacy, University “Ss Cyril and Methodius”, 1000 Skopje, Macedonia
SUZANA TRAJKOVIC JOLEVSKA ; Faculty of Pharmacy, University “Ss Cyril and Methodius”, 1000 Skopje, Macedonia
ANA POCEVA PANOVSKA ; Faculty of Pharmacy, University “Ss Cyril and Methodius”, 1000 Skopje, Macedonia
ANETA DIMITROVSKA ; Faculty of Pharmacy, University “Ss Cyril and Methodius”, 1000 Skopje, Macedonia

Abstract

The stability of proteins is a subject of intense current interest. Aggregation, as a dominant degradation pathway for therapeutic proteins, may cause multiple adverse effects, including loss of efficacy and immunogenicity. In the present study, the formation of aggregates in lenograstim under physiological conditions was monitored. For this purpose, a simple and selective size-exclusion high-performance liquid chromatography method for detection and separation of aggregates from intact protein was developed. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was performed under reducing and non-reducing conditions to determine the nature of aggregate bond formation. Using both techniques, the presence of a low aggregate content attached via disulfide bonds was detected.

Keywords

lenograstim; aggregate formation; size-exclusion high-performance liquid chromatography; sodium dodecyl sulphate-polyacrylamide gel electrophoresis

Hrčak ID:

21049

URI

https://hrcak.srce.hr/21049

Publication date:

1.6.2008.

Article data in other languages: croatian

Visits: 3.176 *