Veterinary Archives, Vol. 89 No. 5, 2019.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.0476
Pathogenicity and pathology of Chryseobacterium sp. PLI2 in experimentally challenged ornamental goldfish, Carasius auratus (L.)
Sudeshna Sarker
; Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India
Thangapalam J. Abraham
orcid.org/0000-0003-0581-1307
; Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India
Gadhadar Dash
; Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India
Talagunda S. Nagesh
; Department of Fisheries Resource Management, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India
Abstract
The pathogenicity and pathology of Chryseobacterium sp. PLI2 in experimentally challenged Carassius auratus goldfish were studied. Chryseobacterium sp. PLI2 produced typical chryseobacteriosis symptoms in intraperitoneally challenged and abrasion-bath treated C. auratus. The LD50 was determined to be 3.13×107 cells/fish. It caused 40% mortality within 3 weeks in abraded fish when challenged at a level of ≈2.50×107 cells/mL. The challenged fish were lethargic, anorectic and exhibited erratic movement. With disease progression, they all had white patches on the gills, excessive mucus secretion, caudal peduncle lesions, focal cutaneous haemorrhages, haemorrhagic eye and opercula, scale loss, skin discoloration, skin peeling, emaciation, pale kidneys and black spleen. Histologically, inflammation of the cartilaginous tissue, fusion of the lamellae and extensive necrosis were noticed in the gills. The spleen showed sinusoidal dilation, melanomacrophage aggregate, marginated leucocytes with a hypertrophied nucleus and necrotized areas. Granuloma-like formations, inflamed renal tubules and glomerulus, extensive necrosis, mild melanomacrophage aggregate, acentric nucleus, constricted renal tubules with a vacuolated surrounding, hypoplastic haematopoietic tissue, degeneration of tubular epithelium with proteinaceous casts in the tubular lumen, and fibrosis around the renal tubules were observed in the kidneys. The challenge experiment indicated that Chryseobacterium sp. can cause systemic disease in goldfish.
Keywords
Carassius auratus; Chryseobacterium sp.; pathogenicity; histopathology; granuloma
Hrčak ID:
228642
URI
Publication date:
27.11.2019.
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