Original scientific paper

https://doi.org/10.24869/psyd.2019.227

ASSOCIATIONS OF GENE VARIATIONS IN NEUROPEPTIDE Y AND BRAIN DERIVED NEUROTROPHIC FACTOR GENES WITH POSTTRAUMATIC STRESS DISORDER

Elma Ferić Bojić ; Department of Genetics and Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina
Sabina Kučukalić ; Department of Psychiatry, Clinical Center University Sarajevo, Sarajevo, Bosnia and Herzegovina
Alma Džubur Kulenović ; Department of Psychiatry, Clinical Center University Sarajevo, Sarajevo, Bosnia and Herzegovina
Esmina Avdibegović ; Department of Psychiatry, University Clinical Center of Tuzla, Tuzla, Bosnia and Herzegovina
Dragan Babić ; Department of Psychiatry, University Clinical Center of Mostar, Mostar, Bosnia and Herzegovina
Ferid Agani ; Faculty of Medicine, University Hasan Prishtina, Prishtina, Kosovo
Miro Jakovljević ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Abdulah Kučukalić ; Department of Psychiatry, Clinical Center University Sarajevo, Sarajevo, Bosnia and Herzegovina
Alma Bravo Mehmedbašić ; Department of Psychiatry, Clinical Center University Sarajevo, Sarajevo, Bosnia and Herzegovina
Emina Šabić Džananović ; Department of Psychiatry, Clinical Center University Sarajevo, Sarajevo, Bosnia and Herzegovina
Nermina Kravic ; Department of Psychiatry, University Clinical Center of Tuzla, Tuzla, Bosnia and Herzegovina
Romana Babić ; Department of Psychiatry, University Clinical Center of Mostar, Mostar, Bosnia and Herzegovina
Marko Pavlović ; Department of Psychiatry, University Clinical Center of Mostar, Mostar, Bosnia and Herzegovina
Branka Aukst Margetic ; Department of Psychiatry, University Hospital Centre Sestre Milosrdnice, Zagreb, Croatia
Nenad Jaksic ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Ana Cima Franc ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Dusko Rudan ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Shpend Haxhibeqiri ; nstitute of Kosovo Forensic Psychiatry, University Clinical Center of Kosovo, Prishtina, Kosovo
Aferdita Goci Uka ; Department of Psychiatry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Blerina Hoxha ; Department of Psychiatry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Valdete Haxhibeqiri ; Department of Biochemistry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Mirnesa Muminović Umihanić ; Community Health Center Zivinice, Zivinice, Bosnia and Herzegovina
Osman Sinanović ; Department of Neurology, University Clinical Center of Tuzla, Tuzla, Bosnia and Herzegovina
Nada Božina ; Department of Laboratory Diagnostics, University Hospital Center Zagreb, Zagreb, Croatia
Christiane Ziegler ; Department of Psychiatry and Psychotherapy, University Hospital Freiburg, Freiburg, Germany
Christiane Wolf ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Wurzburg, Wurzburg, Germany
Bodo Warrings ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Wurzburg, Wurzburg, Germany
Katharina Domschke ; Department of Psychiatry and Psychotherapy, University Hospital Freiburg, Freiburg, Germany
Jürgen Deckert ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Wurzburg, Wurzburg, Germany
Damir Marjanović ; Department of Genetics and Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina

Abstract

Background: Individuals who are exposed to traumatic events are at an increased risk of developing posttraumatic stress disorder (PTSD), a condition during which an individual's ability to function is impaired by emotional responses to memories of those events. The gene coding for neuropeptide Y (NPY) and the gene coding for brain-derived neurotrophic factor (BDNF) are among the number of candidate gene variants that have been identified as potential contributors to PTSD. The aim of this study was to investigate the association between NPY and BDNF and PTSD in individuals who experienced war-related trauma in the South Eastern Europe (SEE) conflicts (1991-1999).
Subjects and methods: This study included participants with current and remitted PTSD and healthy volunteers (N=719, 232 females, 487 males), who were recruited between 2013 and 2015 within the framework of the South Eastern Europe (SEE) - PTSD Study. Psychometric methods comprised the Mini International Neuropsychiatric Interview (M.I.N.I.), the Clinician Administered PTSD Scale (CAPS), and the Brief Symptom Inventory (BSI). DNA was isolated from whole blood and genotyped for NPY rs5574 via PCR - RFLP and NPY rs16147 and BDNF rs6265 using the KASP assay.
Results: Tests for deviation from Hardy-Weinberg equilibrium showed no significant results. Analyses at the categorical level yielded no associations between the affected individuals and all three SNPs when compared to controls. Within lifetime PTSD patients, the major alleles of both NPY variants showed a nominally significant association with higher CAPS scores (p=0.007 and p=0.02, respectively). Also, the major allele of rs5574C>T was associated with higher BSI scores with a nominal significance among current PTSD patients (p=0.047). The results did not withstand a Bonferroni adjustment (????=0.002).
Conclusion: Nominally significant associations between NPY polymorphisms and PTSD susceptibility were found that did not withstand Bonferroni correction.

Keywords

posttraumatic stress disorder; neuropeptide Y; brain-derived neurotrophic factor; war-induced trauma

Hrčak ID:

235150

URI

https://hrcak.srce.hr/235150

Publication date:

9.7.2019.

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