Original scientific paper

https://doi.org/10.24099/vet.arhiv.0411

Genetic polymorphism in the adiponectin (ADIPOQ) gene and its association with the production and reproduction traits of Indian dairy cattle

Vijay Pandey orcid.org/0000-0001-7877-9921 ; Department of Biochemistry, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
Rajesh Nigam ; Department of Biochemistry, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
Satyendra P. Singh ; Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
Deepak Sharma ; Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
Madhu Tiwari ; Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
Satish K. Garg ; Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India

Abstract

The present investigation was performed to explore the SNP in the promoter of the adiponectin (ADIPOQ) gene and its association with production and reproduction traits in Indian Sahiwal cows. A portion of the promoter (ADIPOQ) region of the ADIPOQ gene was amplified which revealed a 977 bp amplicon, and its PCR-RFLP assay with the restriction enzyme TasI revealed three genotypes, in which the CT genotype was the most frequent (62.32%), followed by CC (24.64%) and TT (13.04%). The frequency of the C and T alleles was 0.558 and 0.442, respectively. The ADIPOQ/TasI genotypes revealed a significant association with calving interval (CI) in the first and fourth lactations, in which the CC genotype showed a significantly (P<0.05) longer CI compared to the TT genotype, while in the fourth lactation the TT genotype showed a longer CI compared to the CC genotype. In the fourth lactation, the TT genotype showed a significantly (P<0.05) longer lactation period (LP) and greater total milk yield (TMY) as compared to CC and CT genotypes. In conclusion, the SNP identified in the promoter of the ADIPOQ gene and its association with production and reproduction traits suggests that this gene might serve as a candidate genetic marker for selection of dairy cattle with better milk yield. However, further studies are needed to explore these SNPs in other regions of this gene, and in other breeds and populations.

Keywords

ADIPOQ gene; PCR-RFLP; SNP; reproduction traits; production traits; cattle

Hrčak ID:

235736

URI

https://hrcak.srce.hr/235736

Publication date:

16.3.2020.

Article data in other languages: croatian

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