Veterinary Archives, Vol. 90 No. 1, 2020.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.0438
L-carnitine mitigates bisphenol A-induced hepatic toxicity via activation of Nrf2 and inhibition of pro-inflammatory cytokine gene expression in rats
Mohamed A. Lebda
; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
Aml S. Hashem
; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
Nabil M. Taha
; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
Abd El-Wahab Mandour
; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
Hanan A. Edres
; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
Abstract
Bisphenol, used in many polycarbonate plastics and epoxy resins industries, exerts toxic effects on mammalian organs. The mechanisms underlying bisphenol toxicity are well understood, however the chemoprevention effects of L-carnitine have not yet been recorded. This study aimed to explore the protective mechanism of L-carnitine against BPA-induced hepatotoxicity. Male rats were randomly distributed into 4 groups of 10 rats each: vehicle (5 mL corn oil/kg), bisphenol (50 mg/kg b.w. orally), L-carnitine (500 mg/kg b.w. i/p), and L-carnitine bisphenol pre-treated groups. Bisphenol was dissolved in corn oil and gavaged for 70 consecutive days. Up-regulation of tumor necrosis factor (6.6-fold), and interleukin 6 (3.2-fold) mRNA transcript, along with suppression of nuclear factor erythroid 2-like 2 (0.4-fold), were recorded, following bisphenol administration. Subsequently, bisphenol provoked oxidative stress and attenuated the antioxidative molecules. Finally, hepatic dysfunction was assessed by elevated serum aminotransferases, alkaline phosphatase, lactate dehydrogenase, glutamyl transferase activities and ammonia levels, with the subsequent decline in serum albumin concentration, which confirmed the inflammatory cell infiltration and hydropic degeneration, and the impairment of lipid profile. Interestingly, co-administration of L-carnitine improved liver function and lipid profile, which was explained by the activation of nuclear factor erythroid 2-like 2 (1.5-fold) mRNA transcript, which augmented the antioxidant levels and suppressed oxidative stress, tumor necrosis factor (2.6- fold), and interleukin 6 (1.5-fold) gene expression. In conclusion, L-carnitine exerted hepatoprotective activity against bisphenol toxicity via antioxidant and anti-inflammatory effects.
Keywords
bisphenol A; L-carnitine; pro-inflammatory cytokines; hydropic degeneration; oxidative stress
Hrčak ID:
235740
URI
Publication date:
16.3.2020.
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