Original scientific paper
https://doi.org/10.5599/jese.699
Copper-nickel oxide nanofilm modified electrode for non-enzymatic determination of glucose
Mahsa Hasanzadeh
; Department of Analytical Chemistry, Faculty of Chemistry, University of Urmia, Urmia, Iran
Zahra Hasanzadeh
; Department of Analytical Chemistry, Faculty of Chemistry, University of Urmia, Urmia, Iran
Sakineh Alizadeh
; Faculty of Chemistry, Bu-Ali Sina University, Hamadan, Iran
Mehran Sayadi
; Department of Food Safety and Hygiene, School of Health, Fasa University of Medical Science, Fasa, Iran
Mojtaba Nasiri Nezhad
; Department of Chemical Engineering, Urmia University of Technology, Urmia, Iran
Reza E. Sabzi
; Department of Analytical Chemistry, Faculty of Chemistry, University of Urmia, Urmia, Iran
Sajjad Ahmadi
; Department of Analytical Chemistry, Faculty of Chemistry, University of Urmia, Urmia, Iran
Abstract
CuxO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO4, NiSO4 and H2SO4 for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO4 and NiSO4 was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process.
Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled CuxO-NiO film. The electrocatalytic behavior of the novel CuxO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 μM (S/N = 3) and high sensitivity (1.38 mA mM-1 cm-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared CuxO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.
Keywords
CuxO; NiO; nanofilm; Cu electrode; modifying method; non-enzymatic glucose sensor
Hrčak ID:
238091
URI
Publication date:
19.5.2020.
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