Acta Pharmaceutica, Vol. 71 No. 2, 2021.
Short communication, Note
https://doi.org/10.2478/acph-2021-0014
A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities
OMAR J. PORTILLO-CASTILLO
; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
ROCÍO CASTRO-RÍOS
; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
ABELARDO CHÁVEZ-MONTES
; Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Química, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, México
AZUCENA GONZÁLEZ-HORTA
; Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Ciencias Genómicas, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, México
NORMA CAVAZOS-ROCHA
; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
NOEMÍ WAKSMAN DE TORRES
; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
MARSELA GARZA-TAPIA
; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
Abstract
Recently, pharmaceutical and personal care products (PPCPs) have received considerable attention because of their increasing use. Analysis of PPCPs presents a significant analytical challenge, with high-performance liquid chromatography (HPLC) in reversed-phase mode, as the most widely used analytical technique. To facilitate the optimization of the procedures that are applied in the early stages of sample preparation, a simple and fast HPLC method is proposed in this work for the separation of some PPCPs with a wide range of hydrophilicity. Two columns were evaluated (Atlantis dC18 and Discovery HS F5); as for mobile phases: a formate buffer (40 mmol L-1, pH 4) and methanol were tested in a gradient mode. The fluorinated column allowed better separation in a shorter time and better resolution for all analytes (Rs >1). The proposed method delivered good performance for the tracing of PPCPs and is a suitable alternative to traditional C18-based HPLC methods.
Keywords
pharmaceutical and personal care products; reversed-phase high-performance liquid chromatography, ultraviolet detection
Hrčak ID:
238372
URI
Publication date:
30.6.2021.
Visits: 749 *