Veterinary Archives, Vol. 90 No. 2, 2020.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.0214
Folliculogenesis and follicular fluid adiponectin in cows: its alterations and relationships with ovarian function
Saeedeh Forootan
; Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mehdi Saeb
; Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Amin Tamadon
; The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract
The objectives of the present study were to study the dynamic changes in adiponectin concentration in the growing luteal as well as the preovulatory follicles in dairy cows. In the first study, the ovaries and blood of 15 Holstein dairy cows in the luteal phase were collected from a slaughterhouse. Clear antral follicles were divided into three diameter groups (small, 3-5 mm; medium, 6-9 mm and large, ≥10 mm) and their fluid was aspirated. In the second study, the coccygeal blood and fluid of the preovulatory follicles of eight live Holstein dairy cows were aspirated transrectally, using a transrectal-guided fine-needle. Concentrations of adiponectin in the serum, and follicular fluid and progesterone in the serum were measured. Serum adiponectin concentrations in both luteal and follicular phases were higher than the follicular fluid adiponectin concentrations in all types of follicles (P<0.05); but the trend of a reduction in adiponectin in the serum from the luteal to the follicular phase was similar to the follicular fluid. The follicular fluid adiponectin concentrations of luteal phase follicles in the luteal phase did not differ (P>0.05), and the reduction was seen in preovulatory follicles in comparison with small follicles (P = 0.001). In the luteal phase, a significant positive correlation was observed between the adiponectin concentrations in different sized follicles, and also in the serum progesterone and follicular fluid adiponectin of follicles (P<0.05). In conclusion, lower adiponectin concentrations in blood serum and preovulatory follicles in comparison to luteal growing follicles reflect the effect of ovarian stage on adiponectin alterations.
Keywords
adiponectin; progesterone; follicular fluid; folliculogenesis; cow
Hrčak ID:
238605
URI
Publication date:
25.5.2020.
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