Original scientific paper

https://doi.org/10.24099/vet.arhiv.0563

Genska karakterizacija, kloniranje i ekspresija Toll-like receptora 1 mRNA nilske tilapije (Oreochromis niloticus)

Reham R. Abouelmaatti orcid.org/0000-0001-6777-982X ; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Abdelazeem M. Algammal ; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Wael M. K. Elfeil orcid.org/0000-0001-6777-982X ; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Division of Veterinary Medicine, College of Veterinary Medicine and Animal Science, Jilin University, Changchun, Jilin, China
Noha M. Elshaffy ; Department of Fish Health and Care, Central Lab for Fisheries Research, AL-Abassa, Egypt
Xiaokun Li ; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Jisheng Ma ; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Mohamed Fawzy ; Virology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Ali Wahdan ; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Reham El-Tarabili ; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Iman I. Shabana ; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Biology Department, Faculty of Science, Taibah University, Al- Madinah Al- Munawara, Saudi Arabia

Abstract

Toll-like receptori (TLR) najviše su istraživana skupina receptora za prepoznavanje uzročnika bolesti u kralježnjaka. TLR u riba pokazuju jasna razlikovna svojstva, strukturu i veliku raznolikost u odnosu na druge kralježnjake. Ovo je istraživanje usredotočeno na identifikaciju i otkrivanje Toll-like receptora 1 (TLR1) u nilske tilapije (Oreochromis niloticus) kao predstavnika slatkovodnih riba. Klonirana je puna sekvencija cDNA TLR1 mRNA. Utvrđeno je da se kompletna sekvencija cDNA TLR1 nilske tilapije sastoji od 2355 baznih parova i kodira polipeptid od 785 aminokiselina. Molekularna analiza sekvencija aminokiselina upućuje na to da TLR1 nilske tilapije ima standardna strukturna svojstva i glavne komponente porodice TLR receptora i smatra se ortologom, ne paralogom TLR1 kralježnjaka. Analiza prevedenih aminokiselina pokazala je stupanj identičnost od 96 % s mbuna zebrom, 88 % s lubinom, 85 % s damsel ribom i 85 % s ribom klaun, dok je stupanj identičnosti s električnom jeguljom bio 66 %, a s ribom starlet 61 %. Filogenetska analiza pokazala je da je TLR1 nilske tilapije usko povezan s TLR1 vrsta Larimichthys crocea, Epinephelus coioides i Takifugu rubripes. TLR1 nilske tilapije bio je izražen u bubrezima, mozgu, slezeni, crijevima, mišiću, jetri, škrgama, srcu i na koži. Kvantitativni RT-PCR pokazao je razlike u razini ekspresije među testiranim tkivima. Prema našim podacima ovo je istraživanje prvo koje donosi kompletnu molekularnu i funkcionalnu karakterizaciju Toll-like receptora 1 nilske tilapije, te se smatra funkcionalnim ortologom TLR1 u drugih vrsta.

Keywords

nilska tilapija (Oreochromis niloticus); transmembranska struktura; riba; Toll-like receptor; TLR1; urođena imunost riba

Hrčak ID:

238615

URI

https://hrcak.srce.hr/238615

Publication date:

25.5.2020.

Article data in other languages: english

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