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Croatian Medical Journal, Vol. 59 No. 5, 2018.

Original scientific paper

https://doi.org/10.3325/cmj.2018.59.213

Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades

Anja Kafka ; Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia
Valentina Karin-Kujundžić ; Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia
Ljiljana Šerman ; Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia
Anja Bukovac orcid id orcid.org/0000-0001-6587-8314 ; Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia
Niko Njirić ; Laboratory of Neuro-oncology,Croatian Institute for BrainResearch, University of ZagrebSchool of Medicine, Zagreb, Croatia
Antonia Jakovčević ; Department of Pathology and Cytology, University Hospital Center Zagreb, Zagreb, Croatia
Nives Pećina Šlaus orcid id orcid.org/0000-0002-3334-7671 ; Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia

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Abstract

Aim To identify the involvement of Secreted Frizzled Related
Protein 1 (SFRP1) promoter hypermethylation in different
malignancy grades of astrocytoma and assess its association
with beta-catenin, lymphoid-enhancer factor 1, and
T-cell factor 1.
Methods Twenty-six astrocytoma samples were collected
from 2008-2015. Promoter hypermethylation was evaluated
by methylation-specific polymerase-chain-reaction and
protein expression by immunohistochemistry and stereological
analysis. The staining intensity was scored by comparing
immunoreactivity with normal tissue and by using
10% and 50% cut-offs. Results SFRP1 promoter methylation was found in 32% of
astrocytomas. The number of hypermethylated samples
increased in higher astrocytoma grades and was the highest
in glioblastoma (P = 0.042 compared to other astrocytoma
grades). There was 45.8% of samples with the lack of
or weak expression of SFRP1 protein and 29.2% with strong
expression. Samples with methylated promoter expressed
significantly less SFRP1 than samples with unmethylated
promoter (P = 0.031). Beta-catenin expression levels were
elevated. Yet, glioblastomas with unmethylated SFRP1 promoter
had significantly less beta-catenin (P = 0.033). Strong
expression of lymphoid-enhancer factor 1 was associated
to higher astrocytoma grades (P = 0.006).
Conclusion SFRP1 gene was epigenetically silenced in
glioblastomas when compared to low astrocytoma grades,
which may suggest that the lack of its protein is involved in
astrocytoma progression.

Keywords

Hrčak ID:

239176

URI

https://hrcak.srce.hr/239176

Publication date:

16.10.2018.

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