Original scientific paper
https://doi.org/10.3325/cmj.2019.60.12
Tonsil-derived mesenchymal stem cells exert immunosuppressive effects on T cells
Antun Bačić
; Clinical Department of Ear, Nose and Throat (ENT), Head and Neck Surgery, Merkur University Hospital, Zagreb, Croatia
Drago Prgomet
; University Department of Ear, Nose and Throat (ENT), Head and Neck Surgery, Zagreb University Hospital Center, Zagreb, Croatia
Saša Janjanin
; Dubai Healthcare City, Dubai, United Arab Emirates
Abstract
Aim To assess the immunomodulatory effect of tonsilderived
mesenchymal stem cells (MSCs) on T-lymphocyte
proliferation and cytokine production.
Methods Tonsils were obtained from children aged 3 to
12 years (n = 15) who underwent tonsillectomy for obstructive
sleep apnea from April 2012-October 2014 at the
Merkur University Hospital, Zagreb. Tonsil-derived MSCs
were co-cultured with peripheral blood mononuclear cells
(PBMCs) and phytohemagglutinin as a mitogen. PBMCs
were induced to differentiate into T helper 1 or T helper 2
cells in the presence or absence of tonsil-derived MSCs, after
which the production of interferon-gamma in T helper
1 and interleukin-4 in T helper 2 cells was assessed. Results Tonsil-derived MSC suppressed phytohemagglutinin-
induced proliferation of PBMCs. Compared with controls,
tonsil-derived MSC co-culture significantly decreased
interferon-gamma production (P < 0.001) and increased interleukin-
4 production (P < 0.001).
Conclusion Tonsil-derived MSCs exert immunomodulatory
effects on T lymphocyte proliferation and T helper 1- and
T helper 2-specific cytokine production.
Keywords
Hrčak ID:
239729
URI
Publication date:
15.2.2019.
Visits: 1.018 *