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Chemical and Biochemical Engineering Quarterly, Vol. 34 No. 2, 2020.

Original scientific paper

https://doi.org/10.15255/CABEQ.2020.1783

From Bio-waste to Bone Substitute: Synthesis of Biomimetic Hydroxyapatite and Its Use in Chitosan-based Composite Scaffold Preparation

A. Ressler orcid id orcid.org/0000-0003-2178-5188 ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
A. Gudelj ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
K. Zadro ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Antunović orcid id orcid.org/0000-0002-8233-0059 ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Cvetnić orcid id orcid.org/0000-0002-0168-8092 ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Ivanković orcid id orcid.org/0000-0002-3796-8344 ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
H. Ivanković ; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia

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Abstract

Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have
been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.







This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

biogenic source; chitosan; hydroxyapatite; scaffold; trace element

Hrčak ID:

241912

URI

https://hrcak.srce.hr/241912

Publication date:

31.7.2020.

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