Original scientific paper
https://doi.org/10.15255/CABEQ.2020.1783
From Bio-waste to Bone Substitute: Synthesis of Biomimetic Hydroxyapatite and Its Use in Chitosan-based Composite Scaffold Preparation
A. Ressler
orcid.org/0000-0003-2178-5188
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
A. Gudelj
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
K. Zadro
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Antunović
orcid.org/0000-0002-8233-0059
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Cvetnić
orcid.org/0000-0002-0168-8092
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
M. Ivanković
orcid.org/0000-0002-3796-8344
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
H. Ivanković
; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulićev trg 19, p.p.177, Croatia
Abstract
Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have
been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
biogenic source; chitosan; hydroxyapatite; scaffold; trace element
Hrčak ID:
241912
URI
Publication date:
31.7.2020.
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