Original scientific paper
https://doi.org/10.5599/jese.861
BSA-binding studies of 2- and 4-ferrocenylbenzonitrile: voltammetric, spectroscopic and molecular docking investigations
Hacen Benamara
; University of El Oued, Chemistry Department, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria
Touhami Lanez
orcid.org/0000-0002-3978-7635
; University of El Oued, Chemistry Department, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria
Elhafnaoui Lanez
; University of El Oued, Chemistry Department, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria
Abstract
The binding affinity of 2-ferrocenylbenzonitrile (2FBN) and 4-ferrocenylbenzonitrile (4FBN) with bovine serum albumin (BSA) has been investigated by cyclic voltammetry, absorption spectroscopy and molecular modelling techniques. The results indicated that both of the two derivatives could bind to BSA and cause conformational changes with the order 2FBN > 4FBN. The voltammetric behavior of 2FBN and 4FBN before and after the addition of the BSA suggests that the redox process is kinetically controlled by the diffusion step, and demonstrated that the diffusion coefficients of the 2FBN-BSA and 4-FBN-BSA adducts are lower than that of the free compounds. Furthermore, molecular docking suggested that the binding mode of the two compounds to BSA is of hydrophobic and hydrogen bond interactions, moreover the ligand 2FBN additionally show a π-cation interaction.
Keywords
Hrčak ID:
242259
URI
Publication date:
29.7.2020.
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