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Original scientific paper

https://doi.org/10.2478/acph-2021-0026

Determination of carbohydrates in the herbal antidiabetic mixtures by GC-MC

ALONA SAVYCH ; Department of Pharmacognosy with Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, 46001, Ukraine
SVITLANA MARCHYSHYN ; Department of Pharmacognosy with Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, 46001, Ukraine
IVANNA MILIAN ; Department of General Chemistry, I. Horbachevsky Ternopil National Medical University, Ternopil, 46001, Ukraine


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Abstract

Due to the wide range of biologically active substances, the herbal mixtures can influence the development of diabetes mellitus and its complications. Carbohydrates attract particular attention due to their hypoglycemic, hypolipidemic, anticholesterolemic, antioxidant, anti-inflammatory and detoxifying activities. The aim of this study was to investigate the content of carbohydrates through their monomeric composition in the herbal mixture samples: a) Urtica dioica leaf, Cichorium intybus roots, Rosa majalis fruits, Elymys repens rhizome, Taraxacum officinale roots, b) Arctium lappa roots, Elymys repens rhizome, Zea mays columns with stigmas, Helichrysum arenarium flowers, Rosa majalis fruits, c) Inula helenium rhizome with roots, Helichrysi arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots, d) Cichorium intybus roots, Elymys repens rhizome, Helichrysum arenarium flowers, Rosa majalis fruits, Zea mays columns with stigmas and e) Urtica dioica leaf, Taraxacum officinale roots, Vaccinium myrtillus leaf, Rosa majalis fruits, Mentha piperita herb, which were used in Ukrainian folk medicine for the prevention and treatment of diabetes mellitus type 2.
The carbohydrates were separated by gas chromatography-mass spectrometry after conversion into volatile aldononitrile acetate derivatives. The monomeric composition of polysaccharides was studied after their hydrolysis to form monosaccharides and polyalcohols.
Quantitative analyses of free carbohydrates showed that the predominant sugars were fructose, glucose and disaccharide – sucrose, in all samples. Concerning the determination of polysaccharide monomers after hydrolysis, glucose was the most abundant in all samples. The chromatographic study revealed a number of polyalcohols that are important for the treatment and prevention of progression of diabetes mellitus and its complications, namely, mannitol, pinitol and myo-inositol.

Keywords

carbohydrates; herbal antidiabetic mixtures; GC-MS; monosaccharides

Hrčak ID:

242998

URI

https://hrcak.srce.hr/242998

Publication date:

30.9.2021.

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