Original scientific paper

Light Scattering Determination of the Molecular Weights of the Ammodytes Viper Venom, its Antivenin and Their Aggregates

J. Kratohvil ; Department of Physical Biochemistry, »Andrija Stampar« School of Public Health, Faculty of Medicine, University of Zagreb , Mirogojska 4, Zagreb, Croatia, Yugoslavia
Dj. Ajduković ; Department of Physical Biochemistry, »Andrija Stampar« School of Public Health, Faculty of Medicine, University of Zagreb , Mirogojska 4, Zagreb, Croatia, Yugoslavia

Abstract

A light scattering determination of the molecular weights of the venom of Viperae Ammodytes, its antivenin and their aggregates in 0.15 M sodium chloride solutions were performed. Venom and antivenin were extensively dialyzed against water or sodium chloride solution. The weight-average molecular weight of the venom amounted to 43,600, and of the antivenin (pepsin digested) to 83,000. The size of the venom-antivenin aggregates
depended upon the time of the reaction and the venom-antivenin ratio. The corresponding molecular weights varied from 202 ,000 to 4,800,000. The results were compared with the investigation on similar systems, a nd the significance of the results was discussed.

Keywords

Hrčak ID:

245534

URI

https://hrcak.srce.hr/245534

Publication date:

10.10.1958.

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