Original scientific paper
Substituting Dietary Polyunsaturated Fat with Monounsaturated Fat Increases Insulin Sensitivity in Cultured Rat Hepatocytes
Jagoda Roša
Anja Baraba
Petra Bučević
Darija Đorđijevski
Ivan Antolković
Josip Roša
Abstract
Increased dietary fat intake generally, and saturated fat specifically, will lead to impairment of insulin action and to
development of metabolic syndrome. The aim of this study was to find out changing of hepatic glucose output in dependence
of high monounsaturated and high polyunsaturated fat diet and possible direct action of insulin in hepatocytes.
Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The
glucose production in hepatocytes isolated from rats on high polyunsaturated fat diet, as well as those isolated from rats
on high monounsaturated fat diet was significantly higher than in standard controls. Insulin significantly decreased
glucose production but only in hepatocytes obtained from rats on high monounsaturated fat diet. On the other side energy
expenditure was significantly higher in rats on high monounsaturated fat diet and significantly lower in rats on high
polyunsaturated fat diet comparable with animals on standard diet. Monounsaturated fat in Mediterranean diet could
have beneficial effect on the development of the metabolic syndrome by increasing insulin sensitivity and energy expenditure.
Keywords
insulin; insulin resistance; glucose production; gluconeogenesis; metabolic syndrome
Hrčak ID:
22824
URI
Publication date:
8.5.2008.
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