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Original scientific paper

https://doi.org/10.20471/acc.2020.59.03.02

Pentadekapeptid BPC 157 skraćuje trajanje anestezije rožnice izazvane tetrakainom i oksibuprokainom kod štakora

Ivan Mirković orcid id orcid.org/0000-0002-9462-2067 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Tamara Kralj ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Marin Lozić orcid id orcid.org/0000-0001-5915-626X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Vasilije Stambolija orcid id orcid.org/0000-0003-0670-3965 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Josip Kovačević orcid id orcid.org/0000-0001-7426-201X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Luka Vrdoljak ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Mirna Zlatar ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Kristina Milanović ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Domagoj Drmić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Jurica Predović orcid id orcid.org/0000-0002-4399-3140 ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sanja Masnec ; Department of Ophthalmology, Zagreb University Hospital Centre, Zagreb, Croatia
Matija Jurjević ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Mladen Bušić ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sven Seiwerth ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Antonio Kokot orcid id orcid.org/0000-0002-4836-7536 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Predrag Sikirić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia


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Abstract

U ovom istraživanju ispitivali smo međuodnos anestezije rožnice uzrokovane 0,5% tetrakainom odnosno 0,4% oksibuprokainom i pentadekapeptida BPC 157 (0,4 μg/oko) u kombinaciji s inhibitorom nitrid oksida L-NAME (0,1 mg/oko) i/ili supstratom nitrid oksida L-argininom (2 mg/oko) primijenjenim u obliku kapi za oči. Procjenjivali smo anesteziju rožnice (Cochet-Bonnetov esteziometar), nestajanje lezija rožnice (bojenje 10% fluoresceinom) te volumen nastajanja suza
(Schirmerov test). Učinak potpunog poništavanja anestezije rožnice uočen je u skupinama koje su primale BPC 157. Oporavak je također nastupio u skupinama koje su primale i supstrat i blokator nitrid oksida. L-arginin skraćuje vrijeme neosjetljivosti rožnice, a uočeno je i smanjenje lezija rožnice te poništavanje smanjenja lučenja suza, ali samo u ranijem kraćem razdoblju, dok se kasnije taj učinak gubi. L-NAME je uzrokovao produženje vremena neosjetljivosti rožnice kao i povećanje lezija rožnice te dodatno smanjenje stvaranja suza. Kada se L-arginin i L-NAME daju zajedno njihov učinak se poništava. Opažene razlike mogu ukazivati na određeni utjecaj i uključenost nitrid oksida (neosjetljivost rožnice naspram nastajanja lezija rožnice i stvaranja suza), što je pokazano primjenom supstrata/blokatora nitrid oksida. Međutim, u bilo kojoj kombinaciji prije ili kasnije, dodatak BPC 157 doveo bi do poništavanja učinka primijenjenih anestetika.

Keywords

BPC 157; Tetrakain; Oksibuprokain; Anestezija rožnice; NOS; Štakori

Hrčak ID:

247347

URI

https://hrcak.srce.hr/247347

Publication date:

1.9.2020.

Article data in other languages: english

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