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Original scientific paper

https://doi.org/10.20471/acc.2020.59.03.02

Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats

Ivan Mirković orcid id orcid.org/0000-0002-9462-2067 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Tamara Kralj ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Marin Lozić orcid id orcid.org/0000-0001-5915-626X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Vasilije Stambolija orcid id orcid.org/0000-0003-0670-3965 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Josip Kovačević orcid id orcid.org/0000-0001-7426-201X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Luka Vrdoljak ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Mirna Zlatar ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Kristina Milanović ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Domagoj Drmić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Jurica Predović orcid id orcid.org/0000-0002-4399-3140 ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sanja Masnec ; Department of Ophthalmology, Zagreb University Hospital Centre, Zagreb, Croatia
Matija Jurjević ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Mladen Bušić ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sven Seiwerth ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Antonio Kokot orcid id orcid.org/0000-0002-4836-7536 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Predrag Sikirić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia


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Abstract

We focused on the relationship of 0.5% tetracaine- and 0.4% oxybuprocaine-induced corneal anesthesia in rats, and pentadecapeptide BPC 157 (0.4 μg/eye), along with nitric oxide synthase (NOS) inhibitor N(gamma)-nitro-L-arginine methyl ester (L-NAME) (0.1 mg/eye) and/or NOS substrate L-arginine (2 mg/eye), applied in the form of eye drops. We assessed corneal sensitivity recovery (Cochet-Bonnet esthesiometer), corneal lesion elimination (staining with 10% fluorescein) and decrease in tear volume (Schirmer test). BPC 157 administration had a full counteracting effect. Recovery also occurred in the presence of NOS blockade and NOS substrate application. L-arginine eventually shortened duration of corneal insensitivity and exerted corneal lesion counteraction (and counteraction of tetracaine-induced decrease of tear volume) only in earlier but not in later period. L-NAME application led to longer duration of corneal insensitivity, increase in corneal lesions and decrease in tear volume. When L-NAME and L-arginine were applied together, they antagonized each other’s effect. These distinctions may indicate particular NOS involvement (corneal insensitivity vs. corneal lesion along with tear production), distinctively affected by the administration of NO agents. However, additional BPC 157 co-administration would re-establish counteraction over topical ophthalmic anesthetic-induced effect, be it in its early or late course. We suggest BPC 157 as an antidote to topical ophthalmic anesthetics.

Keywords

BPC 157, Tetracaine, Oxybuprocaine, Corneal anesthesia, NOS, Rats

Hrčak ID:

247347

URI

https://hrcak.srce.hr/247347

Article data in other languages: croatian

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