Original scientific paper

https://doi.org/10.46419/vs.52.4.4

Effects of storage conditions on hematological parameters of Brown bear (Ursus arctos)

Nejra Hadžimusić ; Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Vedad Škapur ; Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Dunja Rukavina ; Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Lejla Velić ; Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Jamin Katica ; Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Abstract

Hematology results are often influenced by the time of blood sampling and analysis, and storage conditions such as temperature during delivery of the sample to the laboratory. Delay of blood analysis may lead to hematological changes that affect interpretation of the data, but may also lead to misdiagnosis. The remote habitats of wild animals, such as bears, pose specific challenges. The aim of this study was to investigate changes in certain hematology parameters (red blood cell count, white blood cell count, packed cell volume, hemoglobin concentration, mean cell (corpuscular) volume, mean cell hemoglobin and mean cell hemoglobin concentration) in the blood samples of brown bears under varying storage conditions (i.e. time and temperature). Blood samples were collected by venipuncture of the jugular vein. Brown bears were sedated prior to collection with combination of metodimidine, tiletamine and zolazepame. Blood samples were stored during 48 hours at two different temperatures (4 °C or 24 °C). Blood samples were collected from nine clinically healthy brown bears of both sexes, aged 3 to 5 years. Hematological determinations were conducted on the blood samples immediately upon collection to obtain the baseline value (BV) and thereafter at specific time intervals (12h, 24h, 36h and 48h) during the storage time for blood samples stored at each temperatures (4 °C or 24 °C). The results showed that brown bear blood samples provided legitimate results for red blood cell count stored at 4 °C for up to 36 hours, and for white blood cell counts stored at 24 °C for up to 24 hours. Legitimate results were obtained for packed cell volume provided at both temperatures for up to 24 hours. Hemoglobin concentration showed significant difference after only 12 hours of
storage at both temperatures. The values of hematological indices determined here reflect the changes of the tested hematological parameters.

Keywords

Brown bear; hematology parameters; duration of storage; temperature

Hrčak ID:

248744

URI

https://hrcak.srce.hr/248744

Publication date:

1.2.2021.

Article data in other languages: croatian

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