Professional paper
DETERMINATION OF THE RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS AGAINST ISONIAZID BY MULTIPLEX PCR METHOD
Mihaela Obrovac
orcid.org/0009-0006-1729-967X
; Institut of Public Health, Zagreb, Croatia
Vera Katalinić-Janković
; Institut of Public Health, Zagreb, Croatia
Magdalena Grce
; Institut “Ruđer Bošković”, Zagreb, Croatia
Abstract
ABSTRACT
Aim: To detect mutations in katG and inhA genes in Mycobacterium tuberculosis (M. tuberculosis) clinical isolates resistant to isoniazid (INH).
Methods: A total of 103 isolates collected from 1999 to 2006 in Croatia and identified as INH-resistant M. tuberculosis strains by standard susceptibility testing proportion method according to Canetti were investigated. Multiplex PCR method was used to detect an AGC to ACC mutation in the katG gene and an inhAC-15T mutation in the regulatory region of the mabA-inhA operon.
Results: Genetic basis of INH resistance was found for 76 (75%) of total 103 M. tuberculosis strains investigated. There was a correlation between mutation in 315 codon of katG gene and acquisition of resistance to other antituberculous drugs.
Conclusions: Mutation in katG gene in INH resistant M.tuberculosis strains is highly predictive for acquisition of resistance to other antituberculous drugs.
Keywords
multiplex PCR; drug resistant tuberculosis; isoniazid; susceptibility testing
Hrčak ID:
23336
URI
Publication date:
20.3.2007.
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