Original scientific paper
https://doi.org/10.15255/CABEQ.2020.1899
Effects of Imprinting and Water Activity on Transesterification and Thermostability with Lipases in Ionic Liquid
M. Matsumoto
orcid.org/0000-0001-6879-1150
; Department of Chemical Engineering and Materials Science, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
K. Nakao
; Department of Chemical Engineering and Materials Science, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
Y. Tahara
; Department of Chemical Engineering and Materials Science, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
Abstract
The effect of bio-imprinting and water activity on catalytic activities and the thermostability of lipases was investigated for transesterification using vinyl acetate and benzyl alcohol as substrates in ionic liquid, [Cnmim][PF6] (n=4,6,8), and benzene. The catalytic activities were enhanced by imprinting in benzene and [C4mim][PF6], and the relations between the transesterification activities and the water activity in both solvents were approximately bell shaped. The reactivity of the transesterification in benzene was higher than that in [C4mim][PF6]. The effects of water activity and imprinting on the kinetic parameters in [C4mim][PF6] were examined. Without controlling the water content, the values of Km,VA and Km,BA (Michaelis constants of vinyl acetate and benzyl alcohol, respectively) decreased, and the values of Vm (maximum rate) increased by imprinting. On the other hand, by controlling the water content in the organic media, the values of Vm, Km,VA, and Km,BA increased by imprinting. The activities of lipase in ionic liquid are more strongly affected by water activity and imprinting than those in benzene. We observed effects of water activity on thermostability but none from imprinting.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
lipase; ionic liquid; bio-imprinting; transesterification
Hrčak ID:
256422
URI
Publication date:
25.4.2021.
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