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Glasnik Zaštite Bilja, Vol. 43. No. 4., 2020.

Original scientific paper

https://doi.org/10.31727/gzb.43.4.7

Bacterial nanocellulose (BNC) biosynthesis, and antimicrobial activity of kombucha in fermented coconut water

Sunčica Beluhan ; Prehrambeno-biotehnološki fakultet, Sveučilište u Zagrebu, Hrvatska
Valerija Nemec ; Prehrambeno-biotehnološki fakultet, Sveučilište u Zagrebu, Hrvatska
Mirela Ivančić Šantek ; Prehrambeno-biotehnološki fakultet, Sveučilište u Zagrebu, Hrvatska

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Abstract

Bacterial nanoellulose (BNC) is a sustainable natural polymeric raw material characterized by exciting properties such as hydrophilicity, chirality, biodegradability, broad chemical-modifying capacity, and the formation of different semicrystalline fiber morphologies. This work studies cultivation of kombucha in aerobic conditions over a period up to 14 days of fermentation in coconut water, at room temperature with different sucrose concentrations added. Changes in pH value, ethanol, acetic acid, gluconic acid, and lactic acid concentrations were observed, as well as yield of BNC. The water release rate (WRR) were investigated, and the results supported its anticipated use as antimicrobial wound dressing material. The antimicrobial activity of kombucha was tested against bacteria Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Bacillus subtilis, as well as yeast Candida albicans. Antimicrobial activitiy of coconut water was observed in the fermented samples against all the investigated bacteria strains, but not completely against yeast Candida albicans.

Keywords

Bacterial nanocellulose, Kombucha, Organic acids, Water release kinetics, Antimicrobial activity

Hrčak ID:

241586

URI

https://hrcak.srce.hr/241586

Publication date:

17.7.2020.

Article data in other languages: croatian

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License (open-access, http://creativecommons.org/licenses/by/2.5/):

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

License (open-access):

Ovo je članak s otvorenim pristupom distribuiran pod uvjetima licence Creative Commons Attribution, koji dopušta neograničenu upotrebu, distribuciju i reprodukciju u bilo kojem mediju, pod uvjetom da se izvorno djelo pravilno citira.

License (open-access):

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Publication date:

Volume: 43

Issue: 4

Pages: 50-62

DOI: 10.31727/gzb.43.4.7

Article Information (continued)

Categories:

Subject: Izvorni znanstveni rad

Categories:

Subject: Original scientific paper

Keywords:

Keyword: bakterijska nanoceluloza, kombucha, organske kiseline, kinetika otpuštanja vode, antimikrobna aktivnost

Keywords:

Keyword: Bacterial nanocellulose, Kombucha, Organic acids, Water release kinetics, Antimicrobial activity

Biosinteza bakterijske nanoceluloze (BNC) i antimikrobna aktivnost fermentirane kokosove vode s kombuchom

Translated Title (en): Bacterial nanocellulose (BNC) biosynthesis, and antimicrobial activity of kombucha in fermented coconut water

Sunčica Beluhan[1]

Email: sunbel@pbf.hr

Valerija Nemec[1]

Email:

Mirela Ivančić Šantek[1]

Email:

 

[1] Prehrambeno-biotehnološki fakultet, Sveučilište u Zagrebu, Hrvatska

Abstract

Bakterijska nanoceluloza (BNC) je obnovljiva prirodna polimerna sirovina koju karakteriziraju izvrsna svojstva poput visoke kristaliničnosti i stupnja polimerizacije, hidrofilnosti, kiralnosti, biorazgradivosti, te sposobnosti stvaranja različitih morfologija polukristaliničnih vlakana. U ovom je radu istraživan uzgoj kombuche u aerobnim uvjetima tijekom 14 dana fermentacije u kokosovoj vodi na sobnoj temperaturi, pri različitim koncentracijama saharoze. Praćene su promjene pH vrijednosti, koncentracije etanola, octene, glukonske i mliječne kiseline, kao i prinos BNC. Istraživana je kinetika otpuštanja vode iz sintetizirane BNC, a rezultati su potvrdili da su uzorci pogodni za zacjeljivanje rana na koži. Antimikrobna aktivnost kombuche je testirana na bakterijama Escherichia coli, Staphylococcus aureus, Salmonella typhimurium i Bacillus subtilis, te kvascu vrste Candida albicans. Uočena je antimikrobna aktivnost fermentirane kokosove vode na bakterije, no ne u potpunosti i na kvasac C. albicans.

Translated Abstract

Bacterial nanoellulose (BNC) is a sustainable natural polymeric raw material characterized by exciting properties such as hydrophilicity, chirality, biodegradability, broad chemical-modifying capacity, and the formation of different semicrystalline fiber morphologies. This work studies cultivation of kombucha in aerobic conditions over a period up to 14 days of fermentation in coconut water, at room temperature with different sucrose concentrations added. Changes in pH value, ethanol, acetic acid, gluconic acid, and lactic acid concentrations were observed, as well as yield of BNC. The water release rate (WRR) were investigated, and the results supported its anticipated use as antimicrobial wound dressing material. The antimicrobial activity of kombucha was tested against bacteria Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Bacillus subtilis, as well as yeast Candida albicans. Antimicrobial activitiy of coconut water was observed in the fermented samples against all the investigated bacteria strains, but not completely against yeast Candida albicans.

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