Original scientific paper

GLYCOSYLATION PATTERNS OF PLACENTAL PROTEINS IN BLIGHTED OVUM

Alan Šerman ; Department of Gynaecology and Obstetrics, General Hospital »Sveti Duh«, Zagreb, Croatia
Ljiljana Šerman ; Faculty of Medicine, Institute of Biology, University of Zagreb, Croatia

Abstract

Blighted ovum is a form of miscarriage, involving the absence or disappearance of an embryo very early in pregnancy. Due to contemporary lack of understanding of the nature of blighted ovum, our objective was to demonstrate the glycosylation patterns of placental proteins in this failure of pregnancy. Six placentas were taken from women with blighted ovum and twentyone placentas were provided from healthy women undergoing elective termination of normal pregnancies. Olygosaccharide branches were detected by Western-blot method using lectins: SNA and PHA-E, after preliminary separation of proteins by discontinuous SDS-PAG electrophoresis. Much stronger expression of GP74 has been identified in blighted ovum at the beginning of the eleventh week of gestation (with PHA-E), than in normal placenta. The same result was obtained for GP25 being stronger in blighted ovum at the end of eleventh week (with SNA). It is possible to conclude that the differences in glycoprotein changes beetwen the blighted ovum and the normal placenta, can only be found at the quantitative level, but not at the qualitative level.

Keywords

glycoproteins; lectins; blighted ovum

Hrčak ID:

23971

URI

https://hrcak.srce.hr/23971

Publication date:

1.12.2006.

Article data in other languages: croatian

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