Veterinary Archives, Vol. 91 No. 3, 2021.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.0674
Genetic diversity within the 18S rRNA and actin locus of Cryptosporidium scrofarum (Apicomplexa: Cryptosporidiidae) infecting domestic pigs (Sus scrofa domesticus) of India
Devina Sharma
; Department of Veterinary Parasitology, DGCN College of Veterinary & Animal Sciences, CSKHPKV, Palampur Himachal Pradesh, India, India; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Nirbhay K. Singh
; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Harkirat Singh
; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Shitanshu S. Rath
; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Damer P. Blake
; Department of Pathobiology and Population Sciences, Royal Veterinary College, North Mymms, Hertfordshire, University of London, United Kingdom
Abstract
The genetic diversity was studied of Cryptosporidium scrofarum (syn Cryptosporidium pig genotype II) of domestic pigs (Sus scrofa domesticus) from Punjab, India. Nested PCR amplification targeting the 18S rRNA and actin gene loci from Cryptosporidium positive samples was carried out, and the amplicons were sequenced. Phylogenetic comparison of a partial 18S rRNA gene revealed that they were genetically most similar to C. scrofarum isolated from other parts of the world. However, comparison of sequences representing a fragment of the genomic actin locus identified a new genotype conserved within the isolates sampled from India but distinct from other published sequences, suggesting the presence of a different Indian genotype.
Keywords
actin; Cryptosporidium scrofarum; genetic diversity; pig; phylogeny; 18S rRNA
Hrčak ID:
260082
URI
Publication date:
9.7.2021.
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