Veterinarska stanica, Vol. 53 No. 3, 2022.
Original scientific paper
https://doi.org/10.46419/vs.53.3.9
Influence of immunomodulators of viral or bacterial origin and vaccine against Aujeszky’s disease on the proportion of peripheral blood B cells in growing pigs
Irena Žarković
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Miroslav Andrišić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Ksenija Šandor
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Anja Vujnović
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Eleonora Perak Junaković
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Branka Šeol Martinec
; Veterinarski fakultet Sveučilišta u Zagrebu, Hrvatska
Marcela Šperanda
; Fakultet agrobiotehničkih znanosti, Osijek, Hrvatska
Hrvoje Valpotić
; Veterinarski fakultet Sveučilišta u Zagrebu, Hrvatska
Andreja Jungić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Miroslav Benić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Luka Cvetnić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Svjetlana Terzić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Abstract
The consequences of infection by Suid herpesvirus type 1 (SuHV-1) that causes Aujeszky’s disease (AD) are well studied, however, the effects of immunomodulators (IMs) of microbial origin (viral and bacterial) when administered solely or in combination with the attenuated SuHV-1 vaccine are less known. The effects of parenteral administration of IMs, inactivated Parapoxvirus ovis (P. ovis) or a combination of inactivated Propionibacterium granulosum (P. granulosum) and detoxified Escherichia coli lipopolysaccharide (LPS) and attenuated SuHV-1, strain Bartha, on the proportion of peripheral blood CD3- CD21+ B cells were analysed in 30 crossbred, 3-month old pigs using flow cytometry (FCM). Specific antibodies for gE and gB of SuHV- 1 were detected using the enzyme-linked immunosorbent assay (ELISA). Data were compared among six experimental groups: (1) pigs that separately received the vaccine, (2) IM of bacterial origin, (3) IM of viral origin, (4) simultaneous administration of the vaccine and bacterial IM, (5) simultaneous administration of the vaccine and viral IM, and (6) the control group of untreated pigs. Comparison of B cell proportions and the detection of specific antibodies in blood samples of vaccinated pigs on Day 11 of the experiment showed a transient decrease in B cell contents, though this could not be assumed to be related since the control group showed a decrease in B cell proportion on the same day. The results showed that the use of IMs alone or in combination with the attenuated SuHV-1 vaccine did not have a significant impact on the proportion of peripheral blood B cells in growing pigs.
Keywords
B cells; pigs; flow cytometry; immunomodulators; Aujeszky disease
Hrčak ID:
263036
URI
Publication date:
31.10.2021.
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