Veterinary Archives, Vol. 91 No. 4, 2021.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.1321
Effects of a radio frequency electromagnetic field on honey bee larvae (Apis mellifera) differ in relation to the experimental study design
Marinko Vilić
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivona Žura Žaja
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Mirta Tkalec
; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Anamaria Štambuk
; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Maja Šrut
; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Goran Klobučar
; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Krešimir Malarić
; Faculty of Electrical Engineering and Computing, University of Zagreb, Zagreb, Croatia
Perica Tucak
; State Inspectorate of Republic of Croatia, Zagreb, Croatia
Selim Pašić
; Department of Physics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Tlak Gajger
orcid.org/0000-0002-4480-3599
; Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Abstract
Exposure to radiofrequency electromagnetic fields (RF-EMF) at the operating frequencies of different communication devices can cause various biological effects. However, there is a lack of studies on the oxidative stress response and genotoxicity in the honey bee (Apis mellifera) after exposure to RF-EMF. In this study, we investigated the oxidative stress and DNA damage in honey bee larvae situated in waxcomb cells, exposed to modulated RF-EMF 23 Vm-1. The glutathione S-transferase activity decreased, whereas the catalase activity increased significantly in the honey bee larvae upon RF-EMF exposure. Superoxide dismutase activity, the level of lipid peroxidation, and DNA damage were not statistically altered in exposed honey bee larvae when compared to the control group. These results suggest that the biological effects of modulated RF-EMF in honey bee larvae depend on the exposure design.
Keywords
antioxidative enzymes; genotoxicity honey bee larvae; oxidative stress; radiofrequency electromagnetic fields
Hrčak ID:
265579
URI
Publication date:
17.11.2021.
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