Original scientific paper

Brzi dokaz vrste Mycobacterium bovis u kliničkim uzorcima uporabom stanične linije THP-1.

Manoharan Parthiban ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Rengarajan Jaiganesh ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Wilson Santhosh Kumar Aruni ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Sundaram Meignanalakshmi ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Pachikani Ramadass ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India

Abstract

Brzo izdvajanje vrste Mycobacterium bovis pokušano je na staničnoj liniji makrofaga THP-1. Stanice THP-1 zaražene tom vrstom pokazivale su karakterističan citopatski učinak 48 sati nakon infekcije. Bakterije su se vrlo dobro razmnožavale već u prvoj pasaži bez potrebe za daljnjim uzgojem. Prisutnost bakterija u hranjivoj tekućini bila je potvrđena bojenjem po Ziehl-Nelseenu i umnožavanjem specifičnih sekvencija ugniježđenom lančanom reakcijom polimerazom.

Keywords

Mycobacterium bovis; stanice THP-1; ugniježđena PCR; Ziehl-Nelseenovo bojenje

Hrčak ID:

24847

URI

https://hrcak.srce.hr/24847

Publication date:

20.6.2007.

Article data in other languages: english

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